非小细胞肺癌PITX1与hTERT mRNA表达相关性分析

Expression of PITX1 mRNA and hTERT mRNA in non-small cell lung cancer tissues

目的:探讨配对同源结构域基因1(PITX1)和人端粒酶逆转录酶(hTERT)基因mRNA在非小细胞肺癌(NSCLC)组织中的表达水平与临床病理因素的关系及其相关性。方法:两步法RT-PCR技术检测91例NSCLC组织和21例支气管扩张或肺大疱等手术切除的良性肺组织中PITX1和hTERT基因mRNA的表达水平。结果:NSCLC组织PITX1基因阳性表达率为24.18%(22/91),明显低于良性肺组织的66.67%(14/21),差异有统计学意义,χ2=14.12,P=0.000。91例NSCLC组织中,hTERT基因过表达72例(79.12%),明显高于其在良性肺组织中的正常表达,χ2=46.52,P=0.000。NSCLC组织PITX1基因mRNA表达与淋巴结有无转移及分化程度(高、中分化与低分化)有关,P值分别为0.004和0.030。hTERT基因mRNA的过表达在不同病理类型中差异有统计学意义,P=0.003。PITX1与hTERT基因的mRNA表达存在相关性,P=0.000。结论:PITX1基因的表达可能对hTERT基因的过表达具有重要的抑制作用;PITX1和hTERT基因mRNA表达状况可能与NSCLC的发生与发展有关。

OBJECTIVE：To explore the expression ievel of PITX1 and hTERT gene mRNA expression in non-small cell lung cancer（NSCLC）. METHODS： The reverse transcription polymerase chain reaction （RT-PCR） was used to detect the PITX1 and hTERT mRNA in 91 cases of non-small cell lung cancer and 21 cases of lung benign diseases tissues sepa- rately. RESULTS： The expression rate of PITX1 gene in non-small cell lung cancer tissues was 24. 18 % （22/91）, which was significantly lower than that in benign lung tissue 66.67% （14/21）, and the difference was statistically significant （X^2= 14.12, P= 0. 000）. hTERT gene overexpression in 72 （79.12 % ） of 91 patients with non-small cell lung cancer, sig- nificantly higher than its normal expression in benign lung tissue,and the difference was statistically significant （^（2^ 46.52, P=0. 000）. The difference was statistically significant in the PITX1 gene mRNA expression in lymph node metas- tasis （P= 0. 004） and the degree of differentiation （high,moderately differentiated and poorly differentiated; P = 0. 030）. hTERT gene mRNA overexpression in pathological type was statistically significant （P=0. 003）. There was correlation between PITX1 gene mRNA and hTERT mRNA expression （P=0. 000）. CONCLUSIONS： PITX1 gene expression may inhibit the over-expression of hTERT gene. mRNA expression of PITX1 and hTERT may be related to the occurrence and development of non-small cell lung cancer.