三七总皂苷促血管内皮细胞增殖的实验研究

Effect of PNS on Proliferation in ECV304 Cells

目的探讨三七总皂苷(panax notoginseng saponins,PNS)对人脐静脉内皮细胞ECV304增殖的影响。方法用不同浓度三七总皂苷对ECV304细胞进行干预培养,采用四唑蓝(MTT)法检测细胞增殖;荧光定量聚合酶链反应法(real-time quantitative polymerase chain reaction,qRT-PCR)检测VEGF、KDR mRNA的表达;Western blot法检测增殖相关蛋白ERK1/2和pERK1/2蛋白的表达。结果三七总皂苷在一定浓度范围(100、200、400、800mg/L)能够促进ECV304细胞增殖(P<0.05),呈明显的剂量依赖性,48h后作用最明显,细胞增殖率分别达112.44%±5.64%、117.20%±11.05%、134.34%±0.40%、141.45%±5.49%;与对照组比较,PNS组ECV304细胞VEGF、KDR mRNA表达量,增殖相关蛋白pERK1/2的表达均明显提高(P<0.05)。结论三七总皂苷能够促进ECV304细胞增殖,其机制可能与上调VEGF、KDR和pERK1/2有关。

Objective To study the effect Panax Notoginseng Saponins （PNS） on proliferation of ECV304 cells. Methods The proliferation of ECV304 cells were evaluated by MTT assay. The expression levels of VEGF and KDR mRNA in ECV304 cells were detected by real - time quantitative polymerase chain reaction. The expression levels of ERK1/2 and pErkl/2 were detected by Western blot. Results Compared with the control group, PNS （100,200,400,800mg/L） promoted the proliferation of ECV304 cells in a dose - de- pendent manner obviously（ P 〈 0.05 ）. The proliferation rates of ECV304 cells induced by 48h PNS were 112.44% ± 5.64% , 117.20%± 11.05% , 134.34% ± 0.40% and 141.45%±5.49 % ,respectively. Compared with the control group, the relative quantity of VEGF ex- pression and KDR mRNA expression in ECV304 cells treated with PNS were increased significantly （ P 〈 0.05 ）. Western blot results showed that PNS increased the expression of proliferation - related protein pERK1/2. Conclusion The PNS can promote the proliferation of ECV30g ceils, the mechanism of which may be related with up - regulating the expression of VEGF,KDR 和 pERK1/2.