六个SMV毒株在大豆不同品种上病毒浓度的变化与比较

THE VIRUS CONCENTRATION OF SIX SMV ISOLATES IN SOYBEAN CULTIVARS

分属於三个株系群的SMV119、245、86—9、87—02、87—44及173毒株,接种到大豆不同品种上,於接种后48小时、10、15、20、25、30、35、40、50、60天用酶联检测植株体内的病毒浓度。结果证明:条件适宜时,接种后48小时各毒株都能在部分顶芽内测出病毒。接种后25天及50为植株体内病毒浓度含量的两个高峰。一个植株内以第一复叶的浓度最高。各毒株以87—02浓度最高,119次之,245最低。病株的病毒浓度并不和外观症状严重程度成正相关。

The virus concentration of 6 SMV - isolates belonging to 3 strain groups was detected by ELISA 2, 10, 15, 20, 25, 30, 35, 40, 50, 60 days after inoculation. And its relation to leaf positsion and symptom severity were also tested. The highest virus concentration appeared 25 days after inoculation.The virus of all the tested SMV isolates could be detected 48hrs after inoculation in the ter-minatl buds. No correlation between severity and virus concentration was found. The second trifoliate from the apical had lowest concentration, and the lower trifoliate near the true leaf had highest.