IL-17、RANTES及其受体在早期自然流产蜕膜组织中的表达及意义

Expression and significance of IL - 17, RANTES and CCR5 in decidua of first trimester spontaneous abortion

目的检测IL-17、T细胞激活上调性表达分泌因子s（RANTES）及其受体CCR5在正常早孕及早期自然流产患者蜕膜组织中的表达，并探讨其意义。方法30例早期自然流产患者（流产组）和30例正常早孕妇女（对照组）的蜕膜组织，分别应用RT—PCR、ELISA方法检测蜕膜组织中IL-17、RANTES及CCR5的表达并进行线性相关分析，同时应用免疫组织化学方法染色定位其蛋白的表达。结果①早孕蜕膜组织中均有IL—17mRNA、RANTESmRNA、CCR5mRNA的表达，流产组蜕膜组织中IL-17mRNA、RANTESmRNA、CCR5mRNA的表达水平高于对照组，差异有统计学意义（P〈0．05）。②ELISA法定量检测结果与PT—PCR结果趋势一致。③IL-17与RANTES的表达呈正相关。④IL-17定位于蜕膜细胞胞质，RANTES定位于蜕膜细胞胞质及胞膜，CCR5定位于蜕膜细胞胞核。结论早期自然流产患者蜕膜组织中IL-17、RANTES及其受体CCR5的表达水平较正常早孕者高，这种改变可能是导致早期自然流产的重要原因。

Objective To investigate the expression of intedeukin - 17 （IL - 17 ）, regulated on activation normal T cell expressed and secreted（RANTES） and CCR5 in decidua of first trimester spontaneous abortion and its significance. Methods Expression of IL - 17, RANTES and CCR5 in decidua of first trimester spontaneous abortion （ abortion group, n = 30） and normal first trimester pregnant women （ control group, n = 30 ） were detected by semi - quantitative RT - PCR, ELISA,and linear correlation analysis was performed. Its protein was detected by immunohistochemistry. Results （1）The IL - 17, RANTES and CCR5 mRNA gene expressions were detected in first trimester decidua tissues, expression of IL - 17, RANTES and CCR5 mRNA was significantly higher in abortion group compared with control group （ P 〈 0.05 ）. （2）The ELISA quantitative test results were consistent with the RT - PCR results. （3）Expression of IL - 17 in de- cidua of first trimester was positively correlated with expression of RANTES. （4）IL - 17 existed in the decidual cell cyto- plasm, RANTES positioning on decidual cell nuclei, CCR5 located in the decidual cell cytosol and membrane. Conclu- sion The higher expression of IL - 17, RANTES and CCR5 in decidua of early spontaneous abortion may be the impor- tant cause leading to spontaneous abortion in early pregnancy.