酶联免疫法检测HBsAg假阳性临床研究

Clinical Research of Enzyme Linked Immunosorbent Assay for The Detection of Hepatitis B Virus Surface Antigen False Positive

目的:研究分析酶联免疫法(ELISA)检测乙肝病毒表面抗原(HBsAg)的假阳性情况。方法:对酶联免疫法检测HBsAg结果为阳性的1500例血清标本用金标法验证,并用化学发光仪微粒子捕捉免疫发光法(MEIA)定量检测HBsAg的含量,以确认酶联免疫法检测结果的假阳性。结果:1500例酶联免疫法检测HBsAg阳性的血清标本,其中1479例为真阳性,真阳性率为98.6%(1479/1500);21例为假阳性,假阳性率为1.4%(21/1500)。结论:酶联免疫法检测HBsAg有一定的假阳性,临床检测时应高度注意,避免错报误诊。

Objective： To study enzyme linked immunosorbent assay （ EI.ISA} in detecting hepatitis B virus surface antigen （HBsAg） of the false positive cases. Method： EHSA detection of HBsAg results for 1500 cases with positive serum samples using the colloidal gold method validation, and using chemical luminous instrument microparticle enzyme lmmunoassay （ ME[A ） for quantitative detection of HBsAg content, to confirm the enzyme-linked immunosorbent assay for detection of false positive results. Result： 1500 cases of enzymeuked immunosorbent assay for detection of HBsAg positive serum samples, including 1479 cases of true positive, true positive rate was 98.6% （1479/1500） ; 21 were false positive, false positive rate was 1.4% （ 21 / 1500 ）. Conclusion： Enzyme-linked immunosorbent assay for detection of HBsAg have false pos- itive, clinical test should be highly attention, avoid the error diagnosis.