小鼠感染日本血吸虫后IL22来源细胞的动态观察

Dynamics of IL-22-producing cells of mice infected with Schistosoma japonicum

目的观察C57BL/6小鼠感染日本血吸虫后,体内细胞因子IL 22来源细胞的动态变化,并分析小鼠体内IL 22的主要来源。方法分别提取感染日本血吸虫0、3、5、8、13周的小鼠淋巴结和脾脏细胞,采用流式细胞术检测各个感染时期IL 22来源细胞的比例。结果感染小鼠的淋巴结和脾脏中,产生IL 22的细胞比例显著升高。在所有产生IL 22的细胞中,有80%左右为T细胞。产生IL 22的CD4-T细胞的比例在感染后3周内明显升高,而产生IL 22的CD4+T细胞的比例到感染第3周后才开始升高,至第8周显著增加。结论日本血吸虫感染小鼠的淋巴结和脾脏中,IL 22由T细胞和非T细胞两大群细胞产生,其中T细胞是细胞因子IL 22最主要的来源。产生IL 22的T细胞分为CD4-T细胞和CD4+T细胞两群,其中产生IL 22的CD4-T细胞在日本血吸虫感染早期迅速增加;而在感染进入慢性期后产生IL 22的CD4+T细胞大量增加。

Objective To study the dynamics of IL-22-producing cells and analyze the main source of IL-22 of mice infected with Schistosoma japonicum. Methods The lymphocytes and splenocytes were prepared from lymphnodes and spleens of mice with S. japonicum infection at different stages （0 w, 3 w, 5w, 8 w, 13 w after infection）. The proportions of IL-22-producing cells were determined by FACS. Results The proportions of IL-22-producing cells increased significantly in the lymphocytes and sple- nocytes from the mice infected with S. japonicum. The IL-22-producing T cells accounted 80% of the total IL-22-producing cells. The IL-22-producing CD4- T cells increased obviously in the 3rd week after S. japonicum infection. However, the proportion of the IL-22-producing CD4 T cells began to rise since the 3rd week post-infection, and increased significantly at the 8th week after in- fection. Conclusion In the lymphnodes and spleens of mice infected with S. japonicum, IL-22 is produced by both T cells and non-T ceils, and T cells are the most important source of IL-22. The IL-22-producing CD4- T cells increase rapidly in the early acute inflammatory response, and the IL-22-producing CD4 T cells increase significantly during the chronic inflammatory phase.