基于均匀设计的海藻玉壶汤中海藻与甘草不同比例配伍对甲状腺肿大大鼠模型肝脏功能及病理形态的影响

Effects of Haizao Yuhu Decoction with different proportions of Sargassum and Radix Glycyrrhizae on hepatic functions and pathomorphology in goiter rats

目的:通过海藻与甘草不同比例配伍的海藻玉壶汤对甲状腺肿大大鼠模型肝功能相关指标的检测及病理形态学观察,考察含"十八反"中海藻、甘草这对反药组合的复方海藻玉壶汤对肝脏系统的影响。方法:将海藻与甘草按照2因素7水平的均匀设计实验原则设置不同配伍比例,灌胃14d,观察海藻与甘草不同配比的海藻玉壶汤对甲状腺肿大大鼠模型肝脏系数、天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、碱性磷酸酶(ALP)、总蛋白(TP)、白蛋白(ALB)、球蛋白(GLB)以及肝组织病理形态的影响。结果:与模型组比较,配比2组(生甘草:海藻为9.68:12.49)、3组(生甘草:海藻为19.36:0.54)、6组(生甘草:海藻为29.04:4.16)ALT水平显著降低(P<0.05);配比3组、6组较模型组AST水平显著降低(P<0.05);配比5组(生甘草:海藻为0.14:8.32)血清中ALP的含量较模型组显著升高,配比5组AST/ALT值显著降低(P<0.05)。结论:含海藻、甘草这对反药组合的复方海藻玉壶汤应用于甲状腺肿大病理模型,在肝脏系统未发现明显的毒副作用,但需进行不同剂量、不同炮制品种的进一步研究方能得出可靠结论。

Objective： To investigate the toxic reactions of Haizao Yuhu Decoction with different proportion of Sargassum and Radix Glycyrrhizae in goiter rats through hepatic functions and pathomorphology. Methods： We set up seven matched groups according to the principle uniform design （tWO factors seven level） compared with normal, model, positive group. At the end of experiment, we determined alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total protein, albumin, globulin by automatic analyzer. The data statistic analysis was processed by ANOVA （analysis of variance） in combination with optimized formula soft. Results： The rats＇ general conditions such as eating, body weight and organization form were not affected; the serum ALT of group 2, group 3 and group 6 had decreased compared with model group; the serum AST of group 3 and group 6 also decreased; compared with model group （P〈0.05）, the serum ALP of group 5 had significantly increased compared with model group （P〈0.05）. AST/ALT ratio of group 5 had decreased compared with model group （P〈0.05）. Conclusion： The drugs, being opposite, may be complementary to each other after compatibility, but should be analyzed synthetically according to different dose and different processed products in order to expound the conclusions of ＇Eighteen Incompatible Medicaments＇.