朱红膏对皮肤破损模型大鼠量毒关系研究

Dose-toxic relationship of Zhuhong Ointment on damaged skin model rats

目的:探讨朱红膏对破损皮肤模型大鼠的量毒关系。方法:随机将大鼠分成对照组、朱红膏低、中、高剂量(19.04、38.08、76.16mg生药/kg)4组。分别于连续给药4周和停药4周后,测定汞含量、β-N-乙酰胺基葡萄糖苷酶(NAG)、碘[125I]β2-微球蛋白(BMG)、丙氨酸转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、肌酐(Scr)、尿素氮(BUN)、血常规、相关凝血指标、脏器系数,同时进行肾脏组织形态学观察。结果:连续给药4周,朱红膏低、中、高剂量组血、尿汞和肾汞含量与对照组比较显著性升高(P<0.01),且存在量毒关系。停药4周,朱红膏低、中、高剂量组血、尿汞含量均恢复正常,肾汞含量仍显著性升高(P<0.01)。高剂量组大鼠肾脏系数及尿NAG显著性升高(P<0.01)。停药4周,不同剂量组与对照组比较没有统计学差异。结论:对破损皮肤模型大鼠长期大剂量使用朱红膏会出现汞蓄积,可能造成肾组织损伤。

Objective： To study the dose-toxic relationship of Zhuhong Ointment on damaged skin model rat. Methods： SD rats were randomly divided into the impairment control group, and high-, middle-, low-dose Zhuhong ointment groups. Each group was treated by corresponding methods for 4 weeks, and recovery 4 weeks. Hematological parameters, kidney and liver function, PT, APTT, NAG and 152-MG contents in urine were taken as monitoring indexes, and the level of mercury in urine, blood and kidney were tested, and the pathological morphology changes of kidney were observed. Results： After treatment for 4 weeks, compared with impairment control group, the level of mercury in urine, blood and kidney in every dose group increased significantly （P〈0.01）. And the relation existed between toxicity and dose on Zhuhong ointment. After recovery for 4 weeks, the level of mercury in urine and blood in every dose group restored normal, while the level of mercury in kidney in high- dose groups still increased （P〈0.01）. The kidney coefficient and the level of NAG increased only in high-dose group （P〈0.01）. There was no significant difference in NAG contents between Zhuhong ointment groups and the impairment control group. Conclusion： Excess using Zhuhong Ointment repeatedly may lead to pathological morphology changes of kidney and abnormal hepatic function, and enhance the accumulation of mercury.