荧光定量PCR法和基因芯片分型法检测人乳头瘤病毒应用对照研究

Fluorescence quantitative PCR method and gene chip parting method in detecting human papillomavirus

目的:探讨荧光定量PCR法和基因芯片分型法对人乳头瘤病毒(HPV)的各自检测效果。方法:选择生殖系病变患者208例,采集其病变组织进行冻存,使用基因芯片分型法进行HPV的检查,并同时对使用基因芯片分型法检查阳性的182例患者的分泌物使用荧光定量PCR法对HPV16,18型进行检测。结果:使用基因芯片分型法检查出的HPV阳性率为87.5%,荧光定量法对HPV18,18型的阳性检查率为79.67%,具有统计学差异;阳性检出率均以22～40岁的患者最多,且在宫颈癌患者中的阳性检出率最高。结论:基因芯片检查法对HPV具有高度的敏感性,荧光定量PCR法对HPV的敏感度较基因芯片检查法低。HPV阳性多发生于22～40岁及宫颈癌患者。

Objective： To explore effect of fluorescence quantitative PCR method and gene chip parting method on detecting human papilloma virus （HPV）. Methods..A total of 208 cases with reproductive diseases were selected. Lesion tissues Were collected and frozen. HPV inspection was performed by gene chip parting method. At the same time HPV16, 18 type detection were performed by fluorescence quantitative PCR method in morbid secretion of 182 cases with positive gene chip result. Results：Gene chip parting method showed that HPV positive rate was 87.5 %, the fluorescence quantitative method showed HPV18, 18 type positive inspection rate was 79.67 %, and the difference was statistically significant; The positive detection rate was highest in 22-40 years old, especially in cervical cancer patients. Conclusions： Gene chip method for HPV is of high sensitivity, while the sensitivity of fluorescence quantitative PCR method for HPV is lower. Cervical cancer patients aged 22-40 years old have the highest HPV positive rate.