激活剂对石油污染土壤修复的强化作用及修复条件的优化

Strengthening Bioremediation of Oil Contaminated Soil by Activation Agent and Optimization of Bioremediation Conditions

在石油污染土壤生物修复实验中,通过添加氮源、葡萄糖、H2O2、木屑4种不同作用的激活剂来强化修复,考察了激活剂的强化修复效果以及各激活剂之间的相互关系,并在单因素实验的基础上,选定氮源、H2O2和木屑的添加量3个影响显著性因素进行响应面优化实验研究,得到最优实验条件,并建立了土壤石油残留率与各激活剂添加量的二次回归方程。结果表明,石油污染土壤生物修复的最佳实验条件为C/N质量比24.6、H2O2的加入量(质量分数)0.32%,木屑加入量2.9%;在此条件下,石油污染土壤强化修复30d后的石油残留率的理论值达43.6%(以修复前土壤样品的石油烃含量为基准),验证值为42.4%,两者相差不大,该模型能用于预测和分析添加激活剂强化修复石油污染土壤的情况。

To strengthen bioremediation of oil contaminated soil, four kinds of activation agents were added to soil, including nitrogen source, glucose, H2O2 and sawdust. The strengthening effects for bioremediation of oil contaminated soil and relations of the four activation agents were studied through experiments. And then based on the result of single factor experiments, nitrogen source, H2O2 and sawdust amount respectively were chosen as the three significant factors most affecting oil degradation for response surface optimization and quadratic regression equation establishment in bioremediation of oil contaminated soil. The results showed that the optimal conditions strengthening bioremediation of oil contaminated soil were mC/mN of 24.6, H2O2 mass fraction of 0.32% and sawdust mass fraction of 2.9%, under which the theoretical value of oil residual rate reached 43.6%（Based on the oil content in oil contaminated soil before bioremediation） by 30 d soil bioremediation, and the validation value was 42.4%. It is clear that the model can be used to forecast and analyze the situation of strengthening bioremediation of oil contaminated soil.