一株新基因型猪圆环病毒Ⅱ型的鉴定

Identification of a Novel Genotype in Porcine Circovirus Type Ⅱ

为了解新近出现的猪圆环病毒Ⅱ型(PCV2)的基因变异及其基因型信息。用PCR技术从新近发生断奶后多系统消耗综合征的病猪组织中扩增和克隆出一株PCV2全基因组序列,并进行了遗传变异分析。结果显示:该PCV2毒株基因组全长为1767bp,与部分其他PCV2毒株的核苷酸同源性达94.0%以上,并显示出一定的地域、时间相关性。病毒ORF2基因系统发育进化分析显示:该PCV2毒株与已有PCV2基因型的遗传距离均大于0.035,因而被划入到一个新的PCV2基因型,暂命名为PCV2d,属于在中国新近出现的一种PCV2基因型。研究结果表明,克隆鉴定了一株新基因型PCV2。

The aim of the present study was to investigate the genetic variation and genotype information of a newly emerging Porcine Circovirus Type 2 （PCV2） strain. The complete genome sequence of one PCV2 strain was amplified and cloned from tissues of pig suffered newly from postweaning multisystemic wasting syndrome by PCR assay. Then, the genetic variation of several PCV2 strains was analyzed. The results showed that the complete genome of this cloned PCV2 strain was 1767 base pair in size, and shared more than 94.0% nucleotide identity with several other PCV2 strains. Moreover, there were some correlation of geography and time among the selected PCV2 strains. The phylogenetic analysis results of the ORF2 genes of these PCV2 strains revealed that the genetic distance between the cloned PCV2 strain and the existed PCV2 genotypes was all more than 0.035, so it was assorted into a novel genotype named PCV2d temporarily, which was a newly emerging PCV2 genotype in China. The research results indicated that one strain of new genotype PCV2 was cloned and identified.