甘蔗葡聚糖酶ScBG1基因的电子克隆及序列分析

In Silico Cloning and Sequence Analysis of Glucanase Gene (ScBG1) from Sugarcane

为了揭示甘蔗β-1,3-葡聚糖酶基因的结构特点和功能特性,从而为该基因的研究和应用提供基础。以水稻β-1,3-葡聚糖酶基因（U72255.1）为搜寻探针,运用电子克隆技术,获得一条甘蔗β-1,3-葡聚糖酶基因全长cDNA,命名为ScBG1,并对其进行生物信息学分析。结果表明：该基因的cDNA序列全长为1842bp,包含一个1488bp完整的开放读码框,编码495个氨基酸残基,属于糖基水解酶第十七家族,为疏水性蛋白,含有信号肽。将ScBG1与其他植物种的β-1,3-葡聚糖酶基因进行序列比对,发现该基因具有保守和典型的葡聚糖酶结构域,碱基序列的保守性为45%~73%。研究结果为该基因的实验克隆奠定基础,也为甘蔗β-1,3-葡聚糖酶基因的分子功能研究提供参考。

In order to reveal the structure and function characteristics of c-l,3-glucanase gene in sugarcane, and provide the basis for the research and application of this gene, a sugarcane full-length eDNA sequence ofβ-l,3-glueanase gene, termed as ScBG1, was obtained in silico by using the ric β-1,3- glucanase gene （U72255.1） as search probe, and the bioinformatics analysis was also finished. The results showed that the full-length of the eDNA sequence of the ScBG1 gene was 1842 bp, which contained a 1488 bp complete open reading frame and encoded 495 amino acid residues. The assemble gene belonged to the 17th glycol hydro family and hydrophobic protein containing the signal peptide. With several plants＇ β-1,3-glucanase Based on the sequences comparison of the ScBG1 gene with β-l,3-glucanase genes from several other plant species, the ScBG1 gene had the typical structural region of β-l,3-glucanase and was high conserved with 45%-73% sequence similarity. The results in this paper provided the basis for experimental isolation of the ScBG1 gene in sugarcane and the reference for the research of its molecule function.