正常肺组织和肺癌组织激光诱发自体荧光光谱

Laser-induced auto-fluorescence spectroscopy of normal lung and lung cancer

目的 分析正常肺组织和肺癌组织的激光诱发自体荧光光谱 ,特征 ,探讨激光诱发自体荧光光谱区分正常肺组织和肺癌组织的可行性。方法 收集肺癌手术标本 39例 ,使用三倍频YAG激光(波长 35 5nm)和光学多道分析仪 (OMA)测定肺癌组织和正常肺组织的激光诱发荧光光谱 ,寻找其自体荧光光谱特征并与病理结果比较。结果 (1)正常肺组织和肺癌组织的主峰位置不同 [(471 6± 5 2 )nm对 (46 1 9± 4 3)nm],向右偏移 (P <0 0 0 1) ;(2 )肺癌组织的相对荧光强度大于正常肺组织 [(2 42 2 7± 434 9)对 (1485 4± 46 2 1) ](P <0 0 0 1) ;(3)正常肺组织在 5 6 0nm及 6 0 0nm处有 2个次峰 ,而肺癌组织平滑下降 ,正常肺组织在 5 80nm与 6 0 0nm的荧光强度比为 0 831± 0 178;肺癌为 1 2 6 9± 0 147(P <0 0 0 1)。结论 正常肺组织和肺癌组织的激光诱发自体荧光光谱有明显区别 ,可用于区分二者。

Objective: To study the feasibility of laser induced auto fluorescence spectroscopy in the differentiation between normal lung tissue and lung cancer. Method: The LIF spectra of normal lung and lung cancer in 39 surgical specimens were examined with a detecting system consisting of an YAG laser(wavelength 355 nm) and an optical multichannel analyzer(OMA), and pathological examinations were done in every testing spots. Results: (1). The location of the principal spectral peaks of the normal lung and lung cancer are different, there is a red shift in the peak emission wavelength in the normal lung tissue [(471.6±5.2)nm] than that of lung cancer[(461.9±4.3)nm](P<0.001). (2). the fluorescence intensity of the lung cancer (24?227±4?349) is higher than that of the normal lung (14854±4621)(P<0.001). (3). In the normal lung tissue spectrum, there are two subsidiary maxima located clearly at 560?nm and 600?nm. The cancer tissue spectrum shows a smoother profile with no subsidiary maxima. The mean fluorescence intensity ratio values at 560?nm and 600?nm were 0.831±0.178 for normal lung tissue and 1.269±0.147 for lung cancer(P<0 001). Conclusion: There is a significant difference in fluorescence spectrum between normal lung tissue and lung cancer, so the LIF spectroscopy may differentiate lung cancer from the normal lung tissue.