芪防膝痹方治疗大鼠膝关节骨性关节炎模型的实验研究

The experimental investigation of Qifangxibi prescription in the treatment of rat knee osteoarthritis model

目的观察芪防膝痹方治疗大鼠膝关节骨性关节炎模型关节软骨的病理改变和血清中PGE2、6-Keto—PGF1α、TXB2的含量变化。方法选取40只4周龄SD大鼠随机分为4组，每组各10只：正常对照组，骨性关节炎模型组，中药干预组，西药干预组。正常对照组不进行处理，普通分笼饲养。余外3组大鼠参照Hulth法造模，中药干预组使用芪防膝痹方灌胃，西药干预组给予西乐葆水溶液灌胃；以上干预自造模建立后第2周开始，2次／d，连续4w。所有大鼠术后第5周时处死并取材，右侧股骨髁标本行藏红-固绿染色观察病理学改变，酶联免疫吸附法测定各组大鼠血清中PGE2、6-Keto—PGF1α和TXB2的表达。结果藏红-固绿染色显示中药干预组关节软骨表面较光滑，表浅层细胞较少，中间层可见细胞簇集现象，潮线紊乱，藏红-固绿染色减退；中药干预组与骨性关节炎模型组比，经芪防膝痹方干预后大鼠膝关节软骨有明显改善，两组间比较差异有统计学意义（P〈0．05）。中药干预组血清中PGE2、6-Keto—PGF1α和TXB2含量低于骨性关节炎模型组，两组间比较差异有统计学意义（P〈0．05）。结论芪防膝痹方改善了大鼠膝关节骨性关节炎模型的病理改变，其机制与抑制相关炎症因子PGE2、6-Keto—PGF1α和TXB2水平有关。

Objective To observe the pathological changes of articular cartilage and changes in serum contents of PGE2, 6-Keto-PGF1 and TXB2 of Qifangxibi prescriPtion in rat knee osteoarthritis model. Methods 40 four-week-old Sprague-Dawley （SD） rats were randomly divided into normal control group, osteoarthritis model group, Qifangxibi prescription group and celebrex group. The rats of normal control group were fed in cages with no interference. The other three groups were developed the model according to Hulth＇s modeling method. Twice a day, the Qifangxibi prescription group was intragastricly administrated Qifangxibi prescription, while the celebrex group was intragastricly administrated celebrex solution since the second week after the models were successfully established. The interference lasts continuously 4 weeks. All rats were executed at the fifth week to observe the pathological changes of the right femoral condylar using Safranine O-Fast Green staining and to detect the expression of serum contents of PGE2, 6-Keto-PGF1 and TXB2 using Biotin-Avidin Enzyme Linked Immunosorbent Assay. Results According to Safranine O-Fast Green staining, the articular cartilage surface of rats in Qifangxibi group was quite smooth with less superficial layer cells and interface cells clustering. Tidal line became disorder and Safranine O-Fast Green staining declined. Compared with the osteoarthritis group, the ar- ticular cartilage of the Qifangxibi prescription group was significantly improved （P 〈 0.05） and the serum contents of PGE2, 6-Keto-PGF1 and＇TXB2 of the Qifangxibi prescription decreased significantly （P〈0.05）. Condusions By the way of in-flammatory factors＇ inhibition of the serum contents of PGE2, 6-Keto-PGF1 and TXB2, the Qifangxibi prescription improves the pathological changes of rat＇s knee oste- oarthritis,