抗人前列腺干细胞抗原单克隆抗体的制备及筛选

Preparation and selection of monoclonal antibodies against human prostate stem cell antigen

目的获得能够结合细胞表面前列腺干细胞抗原(prostate stem cell antigen,PSCA)的抗人PSCA单克隆抗体。方法以重组人PSCA蛋白为免疫原,通过杂交瘤技术制备抗人PSCA单克隆抗体,并进行ELISA、Western印迹、流式细胞术等实验筛选。结果获得6株抗人PSCA单克隆抗体,经过筛选最终得到3株能够结合细胞表面PSCA的单克隆抗体。结论获得具有结合细胞表面PSCA活性的单克隆抗体,为进一步研究和应用奠定了基础。

Objective To prepare and select monoclonal antibodies（mAb） against human prostate stem cell antigens（PSCA） in cells.Methods BALB/c mice were immunized with recombinant human PSCA.Anti-PSCA mAbs were obtained by hybridoma technique,tested by ELISA,Western blotting and flow cytometry.Results Six strains of anti-PSCA mAbs were acquired,three of which proved to be able to bind PSCA in cells after all tests.Conclusion Acquisition of anti-PSCA mAbs able to bind PSCA on the cell surface will facilitate further studies and applications.