金黄色葡萄球菌SarS蛋白参与压力应激的初步研究

SarS protein plays a role in stress response in Staphylococcus aureus

目的通过构建金黄色葡萄球菌(Staphylococcus aureus,简称金葡菌)中SarS蛋白突变株,研究SarS蛋白在金葡菌中的调控功能。方法利用同源重组的方法构建金葡菌8325-4来源的sarS插入突变菌株,通过MDBK细胞模型检测突变株外毒素水平,以及其在高盐、高温等条件下的生长情况。结果成功构建了金葡菌8325-4来源的sarS插入突变菌株。进一步的表型检测结果显示,与野生型菌株相比,sarS突变株在高温、高渗、重金属等应激条件下的生长速度明显下降,但是细菌外毒素对MDBK细胞的杀伤作用没有明显的改变。结论 SarS蛋白在金葡菌抵抗外界压力应激中发挥着重要的功能。

Objective To investigate the regulatory function of Staphylococcus aureus accessory regulator S（SarS） protein in S.aureus via insertion mutant construction.Methods The sarS inactive mutant was constructed by homologous recombination in S.aureus 8325-4.The level of extra-toxins production was detected in MDBK cells,and the growth curves under different stress conditions were measured.Results The sarS mutant was successfully constructed in S.aureus 8325-4.Compared with its parent strain,the level of extra-toxins and the growth of the sarS mutant were not altered.However,it was found that the growth rate of sarS mutant was significantly decreased under several stress conditions compared with its parent strain.Conclusion SarS plays an important role in stress responses in S.aureus.