小动物生物发光活体成像的条件优化与技术扩展研究

Optimization and technology extension of bioluminescence imaging in vivo in small animals

目的为发掘生物发光活体成像技术的灵敏性和应用潜力,进行在体检测条件的优化和技术扩展。方法将稳定高表达荧光素酶的乳腺癌细胞(4T-1-Luc+)和肝癌细胞(Huh-7-Luc+)接种于BALB/c小鼠体内,通过活体成像仪进行生物发光检测灵敏度和毛发的影响分析,利用生物发光结合X线扩展活体成像技术。结果毛发对小鼠皮下接种的肿瘤细胞的生物发光成像有干扰作用,在局部剃除毛发小鼠内体可以检测到50个细胞的光信号值。通过建立X结合发光的检测模型,实现了既能检测发光信号值强度大小,又能比较清晰地观测到部分脏器和发光体(病变组织)的部位。结论实现了生物发光活体成像技术的检测条件优化,建立了X线结合发光新的检测模式,为扩展小动物活体成像的应用范围奠定了基础。

Objective To explore the sensitivity and potential applicability of bioluminescence in vivo imaging technique and to achieve optimization of detection conditions and technique extension.Methods Breast cancer cells 4T-1-Luc＋ or liver cancer cells Huh-7-Luc＋ with stable expression of luciferase were implanted subcutaneously into BALB/c mice.The sensitivity and effect on the hair were investigated using the small animal in vivo imager of bioluminescent signals.Technique extension was performed by combining bioluminescence with X-ray.Results The hair as showed are obvious interference with the detection of bioluminescent signals emitted from the cancer cells implanted in BALB/c mice.The optical signal could be detected in the cellular mass of 50 cells when local hair was removed.The imaging model established by combining X-ray with bioluminescence could not only detect the strength of optical signals emitted,but also clearly locate some organs and the origin of optical signals or pathological tissue.Conclusion Optimization of bioluminescence imaging condition has been achieved.A imaging model of combining X-ray and bioluminescence is established for the purpose of widening the application of small animal in vivo imaging.