水蒸气法提取黑皮油松松针挥发油及GC/MS分析

Volatile Oil Extraction from Pinus tabulaeformis var.mukdensis Uyeke Pine Needle by Vapor Method and Its GC-MS Analysis

为优选黑皮油松挥发油的最佳提取条件,为黑皮油松松针的进一步综合开发利用提供科学依据,采用水蒸气蒸馏法提取马尾松松针挥发油,通过正交试验,研究颗粒大小、液料比、浸泡时间以及提取时间对黑皮油松松针挥发油提取得率的影响进行研究,并用气相色谱-质谱联用技术对其挥发油中化学成分进行分析。结果表明:黑皮油松最佳提取条件为颗粒大小100目,料液比1∶16,浸泡时间16h,提取时间8h;气相色谱-质谱联用技术从黑皮油松松针挥发油中鉴定出23种化合物,占挥发油总量的57.84%,其中烯烃类占25.13%,芳香烃占12.2%,脂类占9.54%,以及少量的醇、酯类化合物。

For the purpose of optimizing the extraction process of volatile oil from the Pinus tabulaeformis var. mukdensis Uyeki pine needle and providing scientific basis for its further development,the volatile oil was ex- tracted from the Pinus tabulaeforrnis var. mukdensis Uyeki pine needle by vapor method. The effects of the particle sides, ratio of material to liquid, soaking time and extracting time on the extraction yield of volatile oil were studied through orthogonal experiment. Then, the chemical constituents of volatile oil were analyzed by gas chromatography-mass spectrometry（GC-MS）. The results showed that the optimum conditions for extrac- ting the volatile oil from the Pinus tabulaeformis var. mukdensis Uyeki pine needle： particle size was 100, ratio of material to liquid was 1：16,soaking time was 16 h,extracting time was 8 h;Gas chromatography-mass spectrometry technology identified 23 compounds, accounting for 57.84% of the total volatile oil,including olefin tired accounted for 25.13%, 12.2% aromatic hydrocarbon,lipid accounted for 9.54%, and a small amount of alcohol, ester compounds.