罗汉果花中黄酮的提取及结构表征

Extraction and Structure Representation of the Flavonoids from Momordica Grosvenorii Flowers

以罗汉果花为原料,采用微波萃取技术提取罗汉果花中的黄酮。探讨了微波功率、微波辐射时间、料液比等因素对黄酮得率的影响。通过正交实验L9(34)对提取工艺进行优化。通过红外光谱、紫外光谱对提取物的结构进行表征。研究结果表明:微波萃取罗汉果花中黄酮的最佳工艺条件为:溶剂60%乙醇溶液,料液比(g/mL)1∶30,微波功率350W、微波辐射时间20min,提取次数2次。在此条件下罗汉果花中黄酮的得率达7.6%。罗汉果花中的黄酮类化合物主要成分为山奈酚。

The flavonoids were extracted from momordica grosvenorii flowers using Microwave- Assisted Extraction. The effects of microwave power, microwave irradiation time, material- liquid ratio and other factors on the yield of the flavonoids from momordica grosvenorii flowers were studied. The extraction conditions were optimized by orthogonal experiment L9 （34）. Infrared spectroscopy and ultraviolet spectroscopy were used to characterize the structure of the extracts. The experimental results have shown that：the optimum conditions of the flavonoids extraction from the momordica grosvenorii flowers using Microwave-Assisted Extraction were solvent 60% ethanol solution,the solid- liquid ratio（g/mL）1 ＂ 30,microwave power 350W,microwave radiation time of20min and extraction 2 times. Under this condition,the flavonoids yield from momordica grosvenorii flowers can reach 7.6 %. The main component of the flavonoids in momordica grosvenorii flowers is kaempferol.