摘要
目的探讨微小RNA（miRNA）在胃癌组织中的表达及其临床意义。方法从52例福马林固定后石蜡包埋（FFPE）的胃癌组织样本中筛选鉴定出可以作为预后标志物的miRNA。从胃癌患者FFPE肿瘤组织样本和正常对照组织中分别提取出总RNA，应用实时定量逆转录聚合酶链反应（RT-qPCR）法检测候选miRNAs的表达水平，并结合临床资料分析评估其在晚期胃癌预后判断中的意义。结果miR-181b在胃癌组织中表达水平显著高于正常胃组织（P〈0．01）。多因素生存分析显示：miR-181b高表达胃癌患者总生存期显著低于miR-181b低表达患者，中位生存期分别为5．03（1．34-8．72）和8．36（7．18～9．55）个月。miR-181b低表达胃癌患者死亡风险HR为miR-181b高表达患者的0．164倍（P〈0．05）。二疗程后疗效与晚期胃癌患者总生存期明显相关。稳定（SD）、进展（PD）患者的死亡风险比（HR）分别为部分缓解（PR）患者的0．552倍（P〉0．05）和3．363倍（P〈0．05）。结论胃癌组织中存在miRNA的异常表达，其中miR-181b高表达与晚期胃癌不良预后显著相关。
Objective To investigate the expression and clinical significance of microRNA （miRNA） in gastric cancer. Methods The expression of candidate miRNAs was quantified from fifty-two advanced gastric cancer FFPE specimens. Total RNAs were extracted from normal and gastric tumor specimens. The levels of candidate miRNAs expression were quantified using real-time reverse transcriptase-polymerase chain reaction （RT-qPCR） analysis. In addition, the prognostic significance in advanced gastric cancer will be further evaluated by combining with clinical data analysis. Results The expression of miR-181b was significantly overexpressed in gastric tumors compared to normal gastric tissues （P 〈 0. 01 ）. Kaplan-Meier survival analysis revealed that high level of miR-181b expression was closely associated with poor overall survival （OS）. The median OS of miR-181b low expression patient subgroup was 8.36 （7. 18- 9. 55） months, while was only 5.03. （1.34-8.72） months for the high expression subgroup. Patients with low miR-181b expression showed a significant decrease in hazard ratio （HR） for death （HR =0. 164, P 〈 0. 05）. The efficacy after two cycles was also found to be significantly correlated with the OS of advanced gastric cancer patients. Compared to patients with patial response （PR） , the HR for death were 0. 552 （ P 〉 0. 05 ） and 3. 363 （ P 〈 0. 05 ） for patients with stable disease （SD） and progressive diease （PD）. Conclusion Abnormal expression of miRNAs has been implicated in gastric cancer, and high expression of miR-181b was closely associated with poor prognosis of advanced gastric cancer patients.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第5期947-950,共4页
Chinese Journal of Experimental Surgery
基金
基金项目：国家自然科学基金资助项目（81171653、30972703）
江苏省自然科学基金资助项目（BK2011246、BK2011247）
常州市社会发展计划基金资助项目（CE20125017、CJ20112020、CS20102020）
常州市科技基础设施滚动计划项目（2010148）
常州市国际科技合作计划项目（CZ20110024）
江苏省科技型企业技术创新基金项目（BC2012093）
