胃癌区域转移淋巴结中锌指蛋白139与基质金属蛋白酶-2、基质金属蛋白酶-7、基质金属蛋白酶组织抑制剂-1表达的关系及意义

Relationship between zinc finger protein 139 with matrix metalloproteinase-2, matrix metalloproteinase-7 and tissue inhibitor of metalloproteinase-1 in local lymph node metastases of gastric cancer

目的检测锌指蛋白139（ZNF139）与基质金属蛋白酶（MMP）-2、MMP-7、基质金属蛋白酶组织抑制剂（TIMP）-1在胃癌原发灶、区域淋巴结转移灶中的表达，并探讨其意义。方法应用逆转录-聚合酶链反应（RT—PCR）及免疫组织化学链菌素抗生物素蛋白-过氧化物酶（SP）法检测46例进展期胃癌患者的原发灶和淋巴结转移灶中ZNF139、MMP-2、MMP-7、TIMP-1mRNA和蛋白水平。结果胃癌区域淋巴结转移灶中ZNF139、MMP-2、MMP-7mRNA表达分别为0．63±0．09、0．75±0．11、0．73±0．11，蛋白阳性率分别为80．43％、84．78％、86．96％，均明显高于原发灶的mRNA（0．32±0．05、0．27±0．04、0．21±0．03）和蛋白阳性率（54．35％、52．17％、58．70％）；而转移灶TIMP-1mRNA（0．63±0．09）和蛋白阳性率（43．48％）则明显低于原发灶（1．24±0．18、78．26％，P〈0．05）。ZNF139与MMP-2、ZNF139与MMP-7的在原发灶中mRNA表达均呈正相关（r分别为0．3258、0．3446，P〈0．05），ZNF139与TIMP-1无明显相关（P〉0．05）；ZNF139与MMP-7在淋巴结中mRNA表达水平呈正相关（r=0．3866．P〈0．05），而ZNF139与MMP-2、ZNF139与TIMP-1无明显相关（P〉0．05）。结论ZNF139可能通过调节部分侵袭相关基因的表达参与了胃癌的侵袭转移。

Objective To investigate the expressions and significance of zinc finger protein 139 （ZNF139） , matrix metalloproteinase （MMP） -2, MMP-7, tissue inhibitor of metalloproteinase （TIMP） -1 in primary tumors （PT） and local lymph node metastases （LNMs） of gastric carcinoma. Methods The expression levels of ZNF139, MMP-2, MMP-7 and TIMP-1 were detected by using reverse transeriptasepolymerase chain reaction （RT-PCR） and immunohistochemistry in 46 paired PT and LNMs of gastric cancer. Results The expression levels of ZNF139, MMP-2 and MMP-7 mRNA （0. 63 ± 0. 09, 0. 75 ± 0. 1 1, 0. 73 ± 0. 11 ） and positive rate of proteins （80. 43% , 84. 78% , 86. 96% ） were all higher in LNMs than the mRNA expression levels （0. 32 ±0. 05, 0. 27 ±0. 04, 0. 21 ±0. 03） and positive rate of proteins （4. 35% , 52. 17% , 58.70% ） in PT, whereas the expression of TIMP-1 mRNA and positive rate of protein were lower in LNMs （0. 63 ± 0. 09, 43.48% ） than in PTs （ 1.24 ± 0. 18,78.26% ） （ all P 〈 0. 05 ）. In PT there was a positive correlation between ZNF139 and MMP-2 or MMP-7 mRNA （r = 0. 3258, 0. 3446, both P 〈 0. 05 ）. In LNMs, positive correlation was found between ZNF139 and MMP-7 （r = 0. 3866,P 〈 0. 05）. Conclusion ZNF139 may be invovled in invasion and metastasis of gastric cancer by regulting some genes.