人脑胶质瘤组织促红细胞生成素受体的表达及其与新生血管的关系

Expression of erythropoietin receptor and its relation with neovascularization in gliomas

目的观察人脑胶质瘤组织促红细胞生成素受体（EPOR）的表达，探讨EPOR与病理分级及新生血管的关系。方法应用组织芯片技术检测152例脑胶质瘤及20例正常脑组织中EPOR及CD105的表达；应用实时荧光定量聚合酶链反应（FQ-PCR）技术检测21例脑胶质瘤及6例正常脑组织中EPOR mRNA的表达。结果64例（42．1％）胶质瘤EPOR表达阳性，2例（10％）正常脑组织表达阳性，差异有统计学意义（χ^2=7．7038，P〈0．01）。EPOR表达强度与病理分级呈正相关（rs=0．3151，P〈0．01）。EPOR高表达组（＋＋、＋＋＋）的CD105计数高于低表达组（-、＋，P〈0．05）。胶质瘤组EPOR mRNA表达与正常组差异无统计学意义。结论人脑胶质瘤组织EPOR的表达在翻译水平上调且与病理级别及新生血管呈正相关。在转录水平未出现上调。

Objective To investigate the expression of erythropoietin receptor （EPOR） in human gliomas and its relationship with histopathological degree and neovascularization. Methods Immunohistochemistry of the tissue microarray was used to detect the expression of EPOR and CD105 in gliomas. Tissue samples included 152 gliomas of different WHO grades and 20 normal brain tissue specimens. Twenty-one gliomas and 6 normal brain tissue specimens were detected by using real-time fluorescent quantitative polymerase chain reaction （FQ-PCR）. Results The expression of EPOR was significantly higher in glioma specimens than in normal brain tissues （χ2 = 7. 7038 ,P 〈 0. 01 ） , and EPOR expression was positively correlated with the stage of the tumor （r, = 0. 3151 ,P〈0. 01 ）. CD105 counts of EPOR （ ＋＋, ＋＋＋） were greater than those of EPOR（ - , ＋ ） （P 〈0. 05）. There was no significant difference in the EPOR mRNA expression in glioma group and normal brain group. Conclusion The expression of EPOR in human glioma exhibits a positive correlation with histopathological degree and neovascularization. There is no up-regula- tion of EPOR mRNA.