雄激素受体基因对人膀胱癌T24细胞黏附和侵袭力的影响

Effects of androgen receptor gene on adhesion and invasion of human bladder cancer T24 cells

目的观察雄激素受体（AR）基因小干扰RNA（siRNA）对膀胱癌黏附和侵袭力的影响。方法采用AR基因siRNA转染膀胱癌T24细胞，分别应用实时定量聚合酶链反应（Real-time PCR）和Western blot检测AR mRNA和蛋白表达水平的变化。转染T24细胞后用噻唑蓝（MTT）比色法检测细胞黏附性，用Transwell方法检测细胞侵袭能力。结果AR—siRNA成功转染膀胱癌T24细胞后，T24细胞AR基因mRNA和蛋白表达水平明显下调。黏附实验结果显示，AR-siRNA转染组细胞黏附率为（38．7±4．4）％，显著低于空白对照组（P〈0．05）；Transwell实验结果显示，AR—siRNA转染组穿膜细胞数为（30．5±6．7）个，空白对照组穿膜细胞数为（59．2±8．1）个（P〈0．05）。结论AR基因在膀胱癌黏附和侵袭中发挥重要作用，以siRNA转染膀胱癌细胞，可以抑制膀胱癌细胞的黏附和侵袭能力。

Objective To study the effects of androgen receptor （AR） gene small interfering RNA （siRNA） on adhesion and invasion of human bladder cancer cells. Methods Human bladder cancer q＇24 cells with AR expression were transfected with AR-siRNA. The expression of AR mRNA and protein was detected by using real-time quantitative polymerase chain reaction （Real-time PCR） and Western blotting, respectively. The cell adhesion was evaluated by using methyl thiazol tetrazolium （MTF） assay, and inva- sion was examined by Transwell chamber. Results T24 cells with AR expression were successfully transfected with AR-siRNA. The Real-time PCR and Western blotting revealed that the expression of AR mRNA and protein was reduced. The adhesive rate in AR-siRNA group was （38.7 ± 4. 4） %, lower than that in blank control group （P 〈 0. 05 ）. The Transwell results showed that the invasion cells were （30. 5 ± 6. 7 ） and （59. 2 ± 8.1 ） in AR-siRNA and blank control groups, respectively （ P 〈 0. 05 ）. Conclusion AR gene might play an important role in adhesion and invasion of human bladder cancer cells, siRNA targeted AR could effectively inhibit adhesion and invasion of human bladder cancer.