辐射对培养小鼠骨髓基质细胞核因子-κB的影响

Effects of radiation on nuclear factor-kappa B in bone marrow stromal cells

目的 探讨辐射对培养小鼠骨髓基质细胞内核因子 κB(NF κB)的影响。方法 采用免疫组化法及凝胶电泳迁移率实验。结果 免疫组化方法显示正常骨髓基质细胞核的周围表达低水平的NF κB ,8.0Gy60 Co辐射损伤后 1h即见其表达较正常显著升高并出现明显的核移位 ,4h达高峰 ,6h有所回落 ,8h恢复正常 ;凝胶电泳迁移率实验发现正常骨髓基质细胞内存在低水平活性NF κB ,8.0Gy60 Co辐射损伤后 1h即见其活性明显升高 ,4h达高峰 ,8h恢复正常。结论 8.0Gy辐射损伤后培养骨髓基质细胞内NF κB的表达升高并激活 ,这可能与骨髓微环境内基质细胞的抗辐射能力及造血功能的恢复有关。

Objective To study the changes of the microenvironment of bone marrow hematopiesis after exposure to gamma radiation. Methods Immunohistochemistry and electrophoretic mobility shift assay (EMSA) were used. Results The expression of nuclear factor kappa B(NFκB) protein in the bone marrow stromal cells (BMSCs) was elevated after exposure to 8 Gy gamma rays radiation determined with immunohistochemistry. The activity of NFκB in the BMSCs was significantly increased after irradia tion determined with EMSA and it reached the peak 4 hours after irradiation. Conclusion Our findings suggest that nuclear factor kappa B in the BMSCs is involved in the protection of BMSCs and in the reco very of hematopoiesis after exposure to radiation.