气相色谱外标法测定食品中甜蜜素含量方法的改进

An improved method for sodium cyclamate determination in food by external standard method of GC

对GB/T 5009.97——2003中利用气相色谱法测定食品中的甜蜜素含量检测方法进行改进。试样在硫酸介质中与亚硝酸钠反应后,再经NaOH碱性水解,以环己醇为对照品,利用气相色谱外标法测定水解己烷提取液的环己醇含量,从而实现食品中甜蜜素的定性及定量检测。该方法与GB/T5009.97——2003相比具有重现性好、准确性高的优点,精密度(n=5)在4.09%-4.28%,回收率在94.60%-96.22%。

An improved determining method of Sodium Cyclamate in food with Gas-Chromatography in GB/T 5009.97 2003 was studied. The sample reacted with Sodium Nitrite in Sulfuric acid solution, then hydrolysis in sodium hydroxide solution and hexane extraction, the con- tent of Cyclohexanol in hexane phase was determined with the external standard of Gas-Chromatography （GC） with reference substance of Cy- elohexanol. This improved method, which can be used in qualitative and quantitative determination of Sodium Cyclamate in food, has ad- vantage with good reproducibility and high accuracy and avoids the negative deviation and bad reproducibility in original method. The method has the advantages of good repeatability, high accuracy than GB/T 5009.97 2003, precision （n=5） in between 4.09%-4.28%, the recovery rate was 94.6.%-96.22%.