树鼩脑血栓梗塞区局部脑血流的变化

Changes of rCBF in the area of cerebral thrombosis induced by photochemical reaction in shrews

本实验采用光化学方法诱导材鼩（低等灵长类）局部脑血栓形成。静脉注射孟加拉红，用λ ５６０ｎｍ的光束照射树鼩右侧颅骨 １０ｍｉｎ，检测实验后 ４、 ２４、 ７２ｈ中心区和半暗区 ｒＣＢＦ、微区 Ｎ＋、 Ｋ＋、 Ｃａ２＋含量、水含量的改变。结果显示：光化学反应后４ｈ中心区和半暗区ｒＣＢＦ明显降低；同时，Ｎａ＋、Ｃａ２＋含量和水含量明显升高并达峰值（Ｐ＜０．０１）。自４ｈ至７２ｈ中心区和半暗区水含量分别在两个水平保持平衡。实验表明光化学诱导的树鼩血栓性局部脑缺血，梗塞范围固定、重复性好，以之探索脑缺血半暗区及中心区缺血性病理生理改变的特点及治疗“时间窗”的选择具有重要意义。

The animal model of photothrombotic focal cerebral ischemia in tree shrews were produced by irradiating the exposed right skull with a spectrally filtered beam of light following injection of rose bengal (20 mg/Kg b.w). The measurements of rCBF (tissue cubes traced with 99mTc-ECD), sodium, potassium, calcium (atomic absorption spectrophotometry) and water contents(gravity gradient column method)were performed at 4, 24 and 72 hour after photochemical reaction. The results showed that rCBF decreased markedly and sodium, calcium and water contents increased to a maximum level by 4 hour concurrently. From 4h to 24h, water content in the core and penumbra remained elevated at two levels respectively. This study demonstrated that this model had the advantages of stable infarction area and reliable reproducibility. It can be used for studying the pathophsiological features of the core and penumbra, allowing propective approach of the determination of 'time window' in clinical cerebral ischemia treatment.