缩胆囊素和谷氨酸对大鼠大脑皮质细胞内游离钙浓度的影响

Effect of cholecystokinin octapeptide and glutamate on intracellur Ca^(2+) concentration in rat cerebo-conical cells

为探讨胆囊收缩素八肽（ｃｈｏｌｅｃｙｓｔｏｋｉｎｉｎ ｏｃｔａｐｅｐｔｉｄｅ， ＣＣＫ－８）及中毒剂量谷氨酸（ｇｌｕｔａｍａｔｅ， Ｇｌｕ）对大鼠大脑皮质细胞内Ｃａ２＋浓度（［Ｃａ２＋］ｉ）的影响及相互关系，以１０－１０、 １０－８、 １０－６ｍｏｌ／Ｌ等浓度ＣＣＫ－８和１ｍｍｏｌ／Ｌ Ｇｌｕ分别或共同作用于大鼠大脑皮质驯胞，以流式细胞仪（ Ｆｌｏｗ ｃｙｔｏｍｅｔｅｒ， ＦＣＭ）检测［Ｃａ２＋］ｉ，并探讨其变化机制。① １０－１０ ｍｏｌ／ＬＣＣＫ－８可增加［Ｃａ２＋］ｉ（Ｐ＜０．０５）， １０－８、１０－６ｍｏｌ／ＬＣＣＫ－８无此作用；②１０－１０ｍｏｌ／ＬＣＣＫ－８与１ｍｍｏｌ／Ｌ Ｇｌｕ共同作用可增加［Ｃａ２＋］ｉ（Ｐ＜０．０５），而１０－６ｍｏ１／ＬＣＣＫ－８不引起［Ｃａ２＋］ｉ增加；③当抑制细胞内钙库释放时，１０－１０ｍｏｌ／ＬＣＣＫ－８加１ｍｍｏｌＬ Ｇｌｕ不能增加［Ｃａ２＋］ｉ。结果提示ＣＣＫ－８生理浓度时可增加［Ｃａ２＋］ｉ，而高浓度时无此作用；ＣＣＫ－８在增加［Ｃａ２＋］ｉ的同时有拮抗 Ｇｌｕ引起的细胞外钙内流作用。

To observe the effect of cholecystokinin octapeptide and glutamate in toxic dose on the changes of [Ca2+]i in the cerebro-conical cells by flow cytometer(FCM). ①[Ca2+]iwas increased by 10-10mol/L CCK-8 (P<0.05); but was not effected by 10-8mol/L or 10-6mol/L CCK-8; ②[Ca2+]iwas elevated by 10-10mol/L CCK-8 and 1mmol/L glutamate simultaneously (P<0.05); but was not elevated by 10-6 mol/L CCK-8 and 1mmol/L glutamate; ③ During inhibiting the release of intracellular Ca2+store, [Ca2+]i was not enhanced.CCK-8 in physiological concentration may increase [Ca2+]i, but CCK-8 in high concentration has no effect, Except increasing[Ca2+]i 10-6mol/L CCK-8 antagonized the inhibition of extracellular Ca2+inflow induced by Glu.