人白细胞抗原一DPB1基因直接测序分型技术

Typing strategy for HLA-DPB1 gene by direct sequencing

研究建立准确的基于人白细胞抗原一ＤＰＢ１（ＨＬＡ－ＤＰＢ１）核酸序列的基因分型技术，为疾病与ＨＬＡ基因相关性研究及器官、组织移植提供准确的ＨＬＡ基因分型方法。使用第十一届国际组织相容性会议提供的引物，经ＰＣＲ技术扩增、分离相应的基因片段，纯化后用ＰＥ公司四色荧光染料终止剂标记循环测序技术直接测定核酸序列，获得个体基因型序列资料，通过与基同型资料数据库比较确定基因型别。这一技术可以准确地确定个体的 ＨＬＡ－ＤＰＢ１的基因型别并得到核酸序列。为进一步研究奠定了基础。本技术可用于其它类似的研究工作。

This study is to establish an accurate gene typing technique based on HLA-DPB I nucleotide sequence, it may be used in study of the relationship between HLA gene and some disease, provide an accurate HLA gene typing method in organ and tissue transplantation. By using of primers published at the Eleventh international Histocompatibility Workshop, individual gene sequence was obtained by PCR amplification, purified and direct sequencing of PCR products. Through compared with the HLA genotype databank, genotype of individual can be identified. The software for the analysis and comparison was created in C computer language. We have established an gene typing technique according to exon 2 nucleotide sequence of HLA-DPBI. This technique can also be used in other similar study.