响应面法优选刺槐花中刺槐苷提取工艺

Optimization of Extraction Technology for Robinin from Flowers of Robinia pseudoacacia by Response Surface Method

目的:优化刺槐花中刺槐苷的提取工艺。方法:根据Box-Benhnken中心组合设计原理,采用响应面分析法优化乙醇体积分数、液料比、浸提时间及提取次数4个自变量对刺槐苷得率的影响,利用SAS 9.2软件对试验数据进行回归分析。结果:最佳提取工艺为加23倍量76%乙醇浸提2次,每次60 min。刺槐苷平均得率达1.834%,转移率86.88%。结论:优选的工艺条件稳定可行,为刺槐花中刺槐苷的提取提供实验依据。

Objective： To optimize extraction technology of robinin from flowers of Robinia pseudoacacia. Method： According to the design principle of Box-Benhnken,effects of ethanol volume fraction,liquid-material ratio,extraction time and extraction times on extraction rate of robinin were studied by response surface method,meanwhile regression analysis of these experimental data was used by SAS 9.2 software. Result： Optimum extraction conditions were obtained as follows：extracted 2 times with 23 times the amount of 76% ethanol,60 min per time.Under these conditions,the average extraction rate of robinin could be up to 1.834% with the transfer rate of 86.88%. Conclusion： These optimized conditions could provide experimental evidence for extracting of robinin from flowers of R. pseudoacacia.