醋调敷吴茱萸粉末的体外透皮吸收考察

Investigation of in vitro Percutaneous Absorption of Evodia rutaecarpa Powder Dressing with Vinegar

目的:考察吴茱萸粉末醋调敷豚鼠的体外透皮渗透特性。方法:采用改良Franz扩散池,以离体豚鼠皮肤为透皮屏障,通过体外透皮吸收试验,用HPLC检测接收液中吴茱萸碱和吴茱萸次碱的质量浓度,计算累积透过量、渗透速率,建立渗透曲线方程。结果:茱萸碱和吴茱萸次碱的透皮吸收速率分别为0.34,0.19μg.cm-2.h-1,24 h累积透过量分别为7.78,4.42μg.cm-2。结论:醋调吴茱萸粉末中吴茱萸碱和吴茱萸次碱具有良好的体外透皮释放性能,为吴茱萸经皮吸收制剂的研究提供参考。

Objective： To investigate in vitro transdermal permeability of Evodia rutaecarpa powder vinegar dressing in guinea pigs. Method： Modified Franz diffusion cell was adopted,with isolated guinea pig skin as transdermal barrier,through in vitro percutaneous absorption test,the content of evodiamine and rutaecarpine was determined by HPLC,then cumulative amount and permeation rate,in order to obtain penetration curve equation. Result： Transdermal absorption rate of evodiamine and rutaecarpine were 0.34,0.19 μgcm-2h-1,the cumulative amount of them in 24 h were 7.78,4.42 μgcm-2. Conclusion： Evodiamine and rutaecarpine from E. rutaecarpa powder dressing vinegar had good in vitro transdermal absorption characteristics,it could provide a reference for study on percutaneous absorption preparation of E. rutaecarpa.