Plackett-Burman试验设计联合星点设计效应面法优选蜘蛛香中总缬草三酯提取工艺

Optimization of Extraction Technology for Total Valepotriates from Valeriana jatamansi by Plackett-Burman Design Combined with Central Composite Design and Response Surface Methodology

目的:采用Plackett-Burman试验联合星点设计效应面法优选蜘蛛香中总缬草三酯的提取工艺。方法:在单因素试验基础上,通过Plackett-Burman试验筛选主要影响因素;采用星点设计-效应面积法优选蜘蛛香中总缬草三酯提取工艺,以乙醇体积分数、浸泡时间、超声时间为自变量,总缬草三酯含量为因变量,通过对自变量与因变量的完全二次响应曲面的回归拟合,利用三维曲面图直观分析总缬草三酯的最佳提取工艺,并进行预测分析。结果:最佳提取工艺为乙醇体积分数65%,液料比10∶1,浸泡时间3 h,室温超声2次,每次30 min。结论:优选的提取工艺简便、精度更高、重复性好、预测性强。

Objective： To optimize extraction process of total valepotriates from Valeriana jatamansi by Plackett-Burman combined with central composite design-response surface methodology. Method： Based on single factor test,Plackett-Burman test was adopted to screen main influencing factors;Extraction technology of total valepotriates was optimized by central composite design-response surface method,with the concentration of ethanol,soaking time,ultrasound time as independent variables,dependent variable was the content of total valepotriates,through complete quadratic response surface regression fitting of independent variables and the dependent variable,optimum technology was determined by intuitive analysis of 3D surface chart,and predictive analysis was used. Result： Optimum extraction technology was as following：the concentration of ethanol 65%,soaking time 3 hours,liquid-solid 10： 1,ultrasonic extracted 2 times at room temperature,30 minutes per time. Conclusion： This optimized process was simple,accurate,reproducible with good predictability.