SPE-HPLC测定两种止咳合剂中吗啡含量

Content Determination of Morphine in Two Kinds of Cough Mixtures by SPE-HPLC

目的:建立小儿止咳合剂和止咳合剂中吗啡的含量测定方法。方法:采用固相萃取预处理结合HPLC测定,色谱条件为Agilent ZORBAX Eclipse XDB-C8色谱柱(4.6 mm×250 mm,5μm),流动相0.05 moL.L-1磷酸二氢钾溶液-0.002 5moL.L-1庚烷磺酸钠溶液-乙腈(18∶18∶5),检测波长220 nm。结果:吗啡在1.717～12.02 mg.L-1(r=0.999 8)与峰面积呈良好线性关系;小儿止咳合剂的平均回收率99.4%(RSD 2.01%),止咳合剂平均回收率100.9%(RSD 0.36%)。结论:该法简便、准确、重复性好,可作为该制剂的质量控制方法。

Objective： To establish a method for determining the content of morphine in childhood cough mixture and cough mixture. Method： Samples were purified by solid phase extraction column and were determined by HPLC.Chromatography conditions were as followings：Agilent ZORBAX Eclipse XDB-C8 column（4.6 mm×250 mm,5 μm）,the mobile phase of 0.05 molL-1 KH2PO4-0.002 5 molL-1 sodium heptane-acetonitrile （18：18：5）,the detection wavelength 220 nm. Result： Morphine had a good linear relationship in the rang of 1.717-12.02 mgL-1（r=0.999 8）.The average recovery of childhood cough mixtrue was 99.4% （RSD 2.01%）,the average recovery of cough mixtrue was 100.9% （RSD 0.36%）. Conclusion： This established method was simple and accurate with good reproducibility,it could be used to effectively control the content of morphine in childhood cough mixture and cough mixture.