西南山茶种子的化学成分研究

Research on Chemical Constituents of Seeds of Camellia pitardii

目的:研究西南山茶种子的化学成分。方法:采用硅胶柱色谱法、Sephadex LH-20柱色谱等方法进行分离纯化,根据波谱学数据和理化性质进行结构鉴定。结果:从西南山茶种子95%乙醇提取物中分离鉴定了7个化合物,分别为山柰酚-3-O-β-D-葡萄糖基-(6→1)-α-L-鼠李糖基-(3→1)-α-L-鼠李糖苷(1)、山柰酚-3-O-β-D-葡萄糖基-(6→1)-4'''-O-乙酰基-α-L-鼠李糖基-(3→1)-α-L-鼠李糖苷(2)、半乳糖醇(3)、蔗糖(4)、胡萝卜苷(5)、β-谷甾醇(6)、羽扇豆醇(7)。结论:化合物1～7均首次从西南山茶种子中分离得到。

Objective： To study the chemical constituents of the seeds of Camellia pitardii. Method： Chemical constituents were isolated by various chromatographic methods and were identified by physicochemical characters and spectroscopic analysis or comparison with standard compounds. Result： From the EtOH extract of the seeds of C. pitardii, seven compounds were isolated and identified as kaempferol-3-O-[α-L-rhamnopyranosyl-（1→3）-α-L-rhamnopyranosyl-（1→6）-β-D-glucopyranoside]（1）, kaempferol-3-O-[α-L-rhamnopyranosyl-（1→3）-（4＇＇＇＇-acetyl）-α-L-rhamnopyranosyl-（1→6）-β-D-glucopyranoside]（2）, D-galactitol（3）, sucrose（4）, daucosterol （5）, β-sitosterol（6）, lupeol（7）. Conclusion： Compounds 1-7 were obtained from the seeds of C. pitardii for the first time.