三重四级杆串联质谱(LC-MS/MS)法同时测定复方广痛消水提物中5种有效成分的含量

Determination of Five Kinds of Effective Components in Guang Tong Xiao Water Extract by LC-MS/MS

目的:建立同时测定广痛消水提物中延胡索乙素、芍药苷、小檗碱、厚朴酚、和厚朴酚含量的方法。方法:采用LC-MS/MS联用方法。结果:芍药苷、延胡索乙素、小檗碱、厚朴酚、和厚朴酚线性范围分别为0.882 8～113(r=0.999 8),1.543 8～49.4(r=0.999 5),0.904 7～115.8(r=0.999 7),0.881 3～112.8(r=0.999 4),0.165 6～21.2 mg.L-1(r=0.999 9)。加样回收率分别为1.998%,1.14%,3.117%,1.828%,1.596%。结论:该法专属性强、快速灵敏,可用于广痛消水提物中延胡索乙素、芍药苷、小檗碱、厚朴酚、和厚朴酚的含量测定。

Objective： To develop a LC-MS/MS method for the determination of tetrahydropalmatine, paeoniflorin, berberine, magnolol and honokiol in Guang Tong Xiao water extract. Method： To measure the content of the target samples by LC-MS/MS method. Result： tetrahydropalmatine, paeoniflorin, berberine, magnolol and honokiol were all analyzed exactly, The linear ranges were 0.882 812 5-113（r=0.999 8）,1.543 75-49.4（r=0.999 5）,0.904 687 5-115.8（r=0.999 7）,0.881 25-112.8（r=0.999 4）, 0.165 625-21.2 mgL-1（r=0.999 9） respectively. The spike recovery were 1.998%,1.14%,3.117%,1.828% and 1.596%. Conclusion： A sensitive,accuracy and suitable LC-MS/MS method has been developed,and the method can be applied for the detemination of tetrahydropalmatine, paeoniflorin, berberine, magnolol and honokiol in Guang Tong Xiao aqueous extract.