摘要
目的:建立同时测定广痛消水提物中延胡索乙素、芍药苷、小檗碱、厚朴酚、和厚朴酚含量的方法。方法:采用LC-MS/MS联用方法。结果:芍药苷、延胡索乙素、小檗碱、厚朴酚、和厚朴酚线性范围分别为0.882 8~113(r=0.999 8),1.543 8~49.4(r=0.999 5),0.904 7~115.8(r=0.999 7),0.881 3~112.8(r=0.999 4),0.165 6~21.2 mg.L-1(r=0.999 9)。加样回收率分别为1.998%,1.14%,3.117%,1.828%,1.596%。结论:该法专属性强、快速灵敏,可用于广痛消水提物中延胡索乙素、芍药苷、小檗碱、厚朴酚、和厚朴酚的含量测定。
Objective: To develop a LC-MS/MS method for the determination of tetrahydropalmatine, paeoniflorin, berberine, magnolol and honokiol in Guang Tong Xiao water extract. Method: To measure the content of the target samples by LC-MS/MS method. Result: tetrahydropalmatine, paeoniflorin, berberine, magnolol and honokiol were all analyzed exactly, The linear ranges were 0.882 812 5-113(r=0.999 8),1.543 75-49.4(r=0.999 5),0.904 687 5-115.8(r=0.999 7),0.881 25-112.8(r=0.999 4), 0.165 625-21.2 mgL-1(r=0.999 9) respectively. The spike recovery were 1.998%,1.14%,3.117%,1.828% and 1.596%. Conclusion: A sensitive,accuracy and suitable LC-MS/MS method has been developed,and the method can be applied for the detemination of tetrahydropalmatine, paeoniflorin, berberine, magnolol and honokiol in Guang Tong Xiao aqueous extract.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第10期98-102,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81072812)