当归、枳壳挥发油HPLC指纹图谱的研究

Fingerprints of Essential Oil from Angelicae Sinensis Radix and Aurantii Fructus by HPLC

目的:研究不同批次当归、枳壳药材经CO2超临界混合萃取的挥发油的指纹图谱,为科学评价和有效控制其质量提供实验依据。方法:采用高效液相色谱,条件为伊利特ODS 2 C18(4.6 mm×250 mm,5μm)色谱柱,乙腈-水为流动相,梯度洗脱,柱温30℃,流速1.0 mL.min-1,检测波长325 nm。结果:建立了11个不同批次的当归、枳壳混合提取挥发油的指纹图谱,以蒿本内酯峰为参照峰,共标定了13个共有峰,利用化学对照品指认了其中2个色谱峰;该指纹图谱可鉴别当归、枳壳经水蒸汽蒸馏法得的挥发油。结论:方法准确可靠,可用于控制淫藤骨痹康方中的挥发油的质量。

Objective： To establish the HPLC fingerprint of essential oil from different batches of Angelicae Sinensis Radix and Aurantii Fructus extracted with supercritical CO2 fluid mixing extraction, and to evaluate and control the quality of it. Method： The analytical column was a Hypersil ODS2 C18 （4.6 mm×250 mm, 5 μm）; a mixture of acetonitrile-water was used as the mobile phase with gradient elution. Flow rate was 1 mLmin-1 wavelength was set at 283 nm. Column temperature was maintained at 30 ℃. Result： The fingerprint of essential oil from Angelicae Sinensis Radix and Aurantii Fructus obtained by mixing extraction was established. The ligustilide peak was taken as the reference peak. Thirteen common peaks were assigned, and two peaks were confirmed by comparing with reference standards. The fingerprint of essential oil obtained by steam distillation can be identified. Conclusion： The method could be used for the quality control of essential oil from Angelicae Sinensis Radix and aurantii fructus obtained by mixing extraction accurately and reliably.