茵陈蒿汤水提物和醇提物活性组分的差异及对D-GalN诱导大鼠急性肝损伤预防作用的比较

Comparative Study of Yinchenhao Tang Water Extract and Its Alcohol Extract and Their Effects on Rat Liver Injury Model

目的:比较茵陈蒿汤水提物和醇提物对大鼠肝损伤的疗效,并初步探讨二者疗效差异的原因。方法:用TLC初步比较茵陈蒿汤水提物和醇提物的成分差异,HPLC测定茵陈蒿汤水提物和醇提物中栀子苷和大黄素的含量,并以D-氨基半乳糖(D-GalN)复制大鼠肝损伤模型,随机分为正常对照组、模型对照组、乙醇提取物高剂量预防组、乙醇提取物低剂量预防组、水提物高、低剂量组。水提物和醇提物的高、低剂量分别以预防性ig高剂量1.67 g.kg-1、低剂量0.84 g.kg-1给药7 d,对照组正常饮食。8 d后观察提取物对大鼠血清肝损伤指标的影响,对肝组织进行常规HE染色。结果:茵陈蒿汤水提物和醇提物的成分存在差别,但二者均具有肝损伤的保护作用,其中水提物能明显降低模型大鼠血清天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、碱性磷酸酶(ALP)、甘油三酯(TG)、总胆红素(T-Bil)含量(P<0.05,P<0.01),改善了肝脏的炎症病理状态。醇提物对血清AST,ALT,ALP等部分指标有所改善(P<0.05,P<0.01)。但水提取物中栀子苷和大黄素的含量均低于醇提取物。结论:以药效为指标,茵陈蒿汤对D-氨基半乳糖所致大鼠肝损伤的保护作用,以水提取工艺为佳。

Objective： To compare the effects of Yinchenhao Tang water extract and its alcohol extract on rat liver injury model and explore the possible reasons for differences in efficacy. Method： The differences were compared in composition of the two extracts by TLC and determining the content of geniposide and emodin by HPLC. The liver injury model was established with galactosamine,the rats were randomized into natural group,model group,Yinchenhao Tang water extract of high dose group,Yinchenhao Tang water extract of low dose group,Yinchenhao Tang alcohol extract of high dose group,Yinchenhao Tang alcohol extract of low dose group. Rats in medication groups were administrated ig with corresponding herbs, water extract group（1.67,0.84 gkg-1）, alcohol extract group（1.67,0.84 gkg-1）and natural group was given normal diet.After treatment for 8 days,the influence of the treatments on the serum hepatic injury indicators was investigated.Hepatic tissues were detected by HE. Result： There were differences between the compositions of two extracts, but both had the protection effect of liver injury with different intensity,and the level of aspartate aminotransferase（AST）, glutamic-pyruvic transaminase（ALT）,alkaline phosphatase（ALP）,triglycerides（TG）, total bilirubin（T-Bil）in liver of water extract group were significantly lower than those of model group（P〈0.05,P〈0.01）.It can improve the steatosis of liver in rats. Meanwhile,the level of AST,ALT,ALP in serum of alcohol extract group were also significantly lower than those of model group（P〈0.05,P〈0.01）.And the content of geniposide and emodin of water extract were lower than those of alcohol extract. Conclusion： Comparing with ethanol extracts of Yinchenhao Tang, its water extracts had certain advantage of the protective effect on D-galactosamine-induced liver injury in rats according to pharmacodynamics results.