妇科千金片对慢性盆腔炎大鼠血清炎性细胞因子表达的影响

Effect of Fuke Qianjin Tablets on Inflammatory Cytokines in Blood Serum in Rats with Chronic Pelvic Inflammatory Disease

目的:研究妇科千金片对慢性盆腔炎大鼠血清炎性细胞因子表达的影响。方法:采用金黄色葡萄球菌、大肠杆菌及解脲脲原体混合菌接种法建立慢性盆腔炎模型。模型成功后,随机分成妇科千金片低、中、高(0.52,1.04,2.08 g.kg-1)剂量组,中药妇炎康对照组(妇炎康0.41 g.kg-1),假手术组,模型组和空白组7组。治疗21 d后,取外周血及子宫组织,采用ELASA法检测血清白介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)和白介素10(IL-10)表达情况,并取子宫组织观察病理学改变。结果:肉眼观察:与模型组比较,妇科千金片低、中、高剂量组子宫肿胀、充血现象明显减轻,结构较清晰。光镜下观察:妇科千金片低、中、高剂量能抑制大鼠子宫内膜炎症细胞增生,促进其病变上皮细胞增生修复,减轻浆膜充血水肿。与正常组比较,模型组血清IL-1β,TNF-α明显升高(P<0.01),血清IL-10明显降低(P<0.01)。与模型组比较,妇科千金片高、中剂量血清IL-1β,TNF-α均明显降低(P<0.01,P<0.05),血清IL-10明显升高(P<0.01);低剂量血清IL-1β和IL-10分别降低或升高(P<0.05)。结论:妇科千金片可促进受损组织与细胞的形态结构及其生理功能的恢复。其作用机制可能与减少促炎症因子IL-1β,TNF-α的释放,促进抗炎症因子IL-10表达,调节Th1/Th2平衡有关。

Objective： To study the effect of Fuke Qianjin tablets on inflammatory cytokines in blood serum in rats. With chronic pelvic inflammatory disease. Method： The rat chronic pelvic inflammation model was established by mixed bacteria of Staphyloccocus aureus,Escherichia coli and M.urealyticum. The rats were randomly divided into 7 groups. Fuke Qianjin tablets in high,middle and low dosage were used to treat chronic pelvic inflammation model rat for 21 days. The content of blood serum interleukin-1β（IL-1β）,tumor necrosis factor-α（TNF-α）and interleukin-10（IL-10）in chronic pelvic inflammation model rat was measured by enzyme-linked immumsorbent assay（ELASA）.Pathologic change of uterus was measured by a microscope. Result： Visual inspection： the Fuke Qianjin tablets in high, middle and low dosage uterine swelling, hyperemia significantly reduced, the structures were clear. Light microscopic inspection： The Fuke Qianjin tablets in high, middle and low dosage can suppression of inflammatory cells in the rat endometrial hyperplasia, to promote its lesion repair of epithelial cell proliferation alleviates seasonal congestion and edema.Serum expressions of IL-1β, TNF-α in model group were markedly higher than those in normal control group（P〈0.01）. Serum expressions of IL-10 in model group were markedly lower than those in normal control group（P〈0.01）.Compared with model group, the serum expressions of IL-1β, TNF-α were significantly lower in Fuke Qianjin tablets high and middle groups（P〈0.01,P〈0.05）. Serum expressions of IL-10 were markedly higher in Fuke Qianjin tablets high and middle groups （P〈0.01）. The serum expressions of IL-1β and IL-10 in Fuke Qianjin tablets low group were higher and lower than those in model group（P〈0.05）. Conclusion： Fuke Qianjin tablets promote the recovery of morphology and physiological function of damaged tissues and cells. Its mechanism may be associated with reduced promoting inflammatory cytokines IL-1β, TNF-α release to promote the anti-inflammatory cytokine IL-10 expression, regulation of Th1/Th2 balance related.