广西藤茶总黄酮对金黄色葡萄球菌抗菌机制研究

Antibacterial Mechanisms of Total Flavonoids from Ampelopsis grossedentata on Staphylococcus aureus

目的:研究广西藤茶总黄酮(TF)抗金黄色葡萄球菌(SA)作用机制,从而初步明确其作用靶位。方法:使用不同终质量浓度TF(310～38.75 mg.L-1)处理SA,另设不经药物处理的SA为对照,采用扫描电镜法观察155 mg.L-1TF对菌体的超微形态结构,微生物粘着碳氢化合物法测定155 mg.L-1TF对菌体表面疏水性,电导率法测定77.5,155,310 mg.L-1TF对菌体通透性及比色法测定38.75,77.5,155,310 mg.L-1TF对细菌脱氢酶活性的影响。结果:随着药物浓度增加,作用时间延长,细菌菌体超微形态有明显改变,表现为菌体表面粗糙不平,产生瘤状突起,细胞间界限模糊不清,细胞直径变大等;与不经药物处理的SA比较,TF作用SA 4,8,16 h后,能明显降低细菌细胞表面疏水性(P<0.01);明显增加细菌细胞通透性;明显抑制细菌脱氢酶的活性。结论:广西藤茶总黄酮可能通过降低细菌表面疏水性,增加细菌通透性使细菌形态发生改变,并通过抑制细菌脱氢酶活性发挥抗菌作用。

Objective： To investigate the antibacterial mechanisms of total flavonoids （TF） from Ampelopsis grossedentata on Staphylococcus aureus. Method： The antibacterial mechanisms were studied by scanning electron microscope for ultrastructural morphology of TF （155 mgL-1）. Microbial adhesion method was used for determination the effect of TF （155 mgL-1） on cell-surface hydrophobicity of hydrocarbons. Cell permeability and conductivity colorimetric method were employed for the determination of dehydrogenase activity of S. aureus after being treated by different concentrations of total flavone（310-38.75 mgL-1）from Ampelopsis grossedentata of Guangxi. Result： As the concentration increased and time prolonged, bacterial cell ultrastructural change was evident, characterized by cell surface uneven, warty protrusions, intercellular boundaries blurred, cell diameter increase. TF could significantly reduce the bacterial cell-surface hydrophobicity （P〈0.01） and increase in permeability of the bacterial cell. Activity of bacterial dehydrogenase was obviously inhibited by TF. Conclusion： The antibacterial mechanisms of TF from Ampelopsis grossedentata on S. aureus are related with reducing surface hydrophobicity, increasing permeability of the bacterial and inhibiting dehydrogenase activity of S. aureus.