榄香烯注射液对鼻咽癌细胞株CNE1增殖和凋亡的影响

Effects of Elemene Injection on Proliferation and Apoptosis of Nasopharyngeal Carcinoma Cell Line CNE1

目的:探讨榄香烯注射液对鼻咽癌细胞株CNE1增殖和凋亡的影响。方法:分别用20,10,5,2.5,0 g.L-1榄香烯注射液处理CNE1细胞12,24,48 h后,Prestoblue法检测细胞的增殖抑制率,光镜观察细胞形态变化,Hoechst-PI双染、流式细胞仪检测细胞的凋亡。结果:榄香烯注射液对CNE1细胞的增殖有抑制作用,并呈剂量和时间依赖性,处理12,24,48 h的IC50值分别为(10.70±0.26),(7.06±0.11),(5.52±0.10)g.L-1;光镜下显示,给药后细胞大量凋亡,折光性下降;榄香烯注射液可诱导CNE1细胞凋亡。结论:榄香烯注射液能抑制CNE1细胞增殖并诱导其凋亡。

Objective： To study the effect of elemene injection on proliferation and apoptosis of nasopharyngeal carcinoma cell line CNE1. Method： The cytotoxic effect of elemene injection （20,10,5,2.5,0 gL-1： 12,24,48 h） on CNE1 cells was tested by Prestoblue,cell morphological changes was observed by light microscope and apoptosis of CNE1 cells was observed by Hoechst-PI double staining and flow cytometric analysis. Result： Elemene injection showed an inhibitory effect with dose-response relation on CNE1 cells. The IC50 values at 12,24,48 h were （10.70±0.26）,（7.06±0.11）,（5.52±0.10） gL-1 respectively.Light microscope and hoechst-PI double staining showed apoptotic morphology change. Conclusion： Elemene injection can inhibit the proliferation of cell line CNE1 through the induction of apoptosis.