高效液相色谱法测定鱼肉中的碱性橙Ⅱ和碱性嫩黄O两种工业染料

Determination of chrysoidin Ⅱ and auramine O in fish via high performance liquid chromatography

碱性橙Ⅱ和碱性嫩黄O均为工业用着色剂,不能作为食品添加用色素。为此建立了鱼肉样品中同时检查碱性橙Ⅱ和碱性嫩黄O的高效液相色谱定量分析方法,以甲醇-0.03%磷酸水溶液(pH 2.0)为流动相,Hydrosphere-C18色谱柱分离,二极管阵列检测器检测,检测波长为440 nm,可以实现鱼肉样品中添加的碱性橙Ⅱ和碱性嫩黄O的含量分析。该方法碱性橙Ⅱ和碱性嫩黄O的线性范围均为0.1～10μg/mL,最低检出浓度均为0.04μg/mL,方法的回收率为95%～116%,相对标准偏差(RSD)为2.0%～3.5%。此外,还进一步考察了前处理方法中采用磷酸-甲醇直接研磨鱼肉并抽提被分析物,抽提液适当蒸发浓缩,可以实现鱼肉样品中添加0.25μg/g的碱性橙Ⅱ和碱性嫩黄O的分析检测,以及自制浸泡黄鱼样品中的碱性橙Ⅱ和碱性嫩黄O的分析检测。

A method for the determination of ehrysoidin Ⅱ and auramine O in fish was established via high performance liquid chromatography （HPLC）. The separation was per- formed in a YMC hydrosphere-C18 （250 mm ×4.6 mm i. d. 5 μm） column, methanol and 0.03% H3PO4 water solution were used as mobile phase. A photo-diode array detector was used to detect them at 440 nm. The linear range was O. 1 × 1.0 μg=/mL, and the detection limit was 0. 04 μg./mL. The re- covery ranged from 95% to 116% and RSD was 2. 0% -3. 5%. chrysoidin Ⅱ and auramine O were successfully detected at the level of 0.25 μg/g in fish after extracted by H3PO4-meth- anol solution and then concentrated by evaporation.