摘要
综述了当前国内外应用于土壤污染生态研究中的各种分子生物学和系统生物学技术,包括核酸杂交和DNA指纹图谱分析技术以及宏基因组学、宏蛋白质组学和代谢组学等"组学"技术。阐述了这些技术方法的原理、应用以及各自的优势和局限性,并对这些新兴生物技术在土壤污染生态学中的应用前景作一展望。
This paper reviews the current applications of molecular and systematic biological technologies in polluted soil microbial ecology researches. These approaches include nucleic acid hybridization, DNA fingerprints, metagenomics, metaproteome and metabolomics. The mechanisms, applications, advantages and limitations of these approaches were discussed respectively, and the potential applications of these biological technologies were also discussed.
出处
《土壤学报》
CAS
CSCD
北大核心
2013年第3期609-617,共9页
Acta Pedologica Sinica
基金
科技部863计划重大项目(2012AA06A204)
国家自然科学基金项目(41230858
41201313)资助
