摘要
[目的]获取白术中高质量基因组DNA。[方法]选取新鲜白术叶片,破碎细胞壁,用经改良的CTAB与SDS法进行DNA分离纯化提取。[结果]改良CTAB法提取的DNA OD值为1.8～1.9,较改良SDS法的OD值(1.5～1.6)高。[结论]改良CTAB法提取的DNA浓度和纯度较高,可作为模板用于后续分子标记的研究。
[Objective] To obtain high-quality DNA from Atractylodes macroephala.[Method] Selecting fresh leaf of A.macroephala,broken cell wall,CTAB and SDS method were adopted to conduct DNA isolation and purification.[Result] The results showed that OD value by using improved CTAB method is 1.8-1.9,which is higher than OD value 1.5-1.6 by using improved SDS method.[Conclusion] DNA concentration and purification by using CTAB method is higher,which can be used as templet in subsequent molecular markers.
出处
《安徽农业科学》
CAS
2013年第7期2860-2861,共2页
Journal of Anhui Agricultural Sciences
关键词
白术
CTAB
SDS
Atractylodes macroephala
CTAB
SDS
