摘要
[目的]测定怀山药及零余子中尿囊素成分的含量。[方法]以尿囊素为标准对照品,采用HPLC法在波长224 nm处对怀山药及零余子的尿囊素成分进行含量测定。[结果]平均加标回收率为98.35%,RSD为1.29%;怀山药中尿囊素含量为0.533%,怀山药零余子中尿囊素含量为0.848%;零余子中尿囊素含量略高于怀山药。[结论]该方法简便、易行、准确度高,可用于怀山药零余子中尿囊素成分的含量测定。
[Objective] To determine the allantoin content in Dioscorea opposita Thumb.and bulbil.[Method] With allantoin as the control substance,the content of allantoin was measured using HPLC at the detection wavelength of 224 nm.[Result] The content of allantoin in D.opposita and bulbil was 0.533% and 0.848%,respectively.The average recovery rate of allantoin was 98.35% with relative standard deviations of 1.29%.[Conclusion] The method is simple,easy,accurate and suitable for content determination of allantoin in D.opposita and bulbil.
出处
《安徽农业科学》
CAS
2013年第7期2912-2912,2928,共2页
Journal of Anhui Agricultural Sciences
基金
资源植物生物学安徽省重点实验室项目
关键词
怀山药
零余子
尿囊素
HPLC
Dioscorea opposita Thunb.
Bulbil
Allantoin
HPLC