HPLC法测定怀山药零余子尿囊素的含量

Determination of Allantoin Content in Dioscorea opposita Thunb. and Bulbil by HPLC

[目的]测定怀山药及零余子中尿囊素成分的含量。[方法]以尿囊素为标准对照品,采用HPLC法在波长224 nm处对怀山药及零余子的尿囊素成分进行含量测定。[结果]平均加标回收率为98.35%,RSD为1.29%;怀山药中尿囊素含量为0.533%,怀山药零余子中尿囊素含量为0.848%;零余子中尿囊素含量略高于怀山药。[结论]该方法简便、易行、准确度高,可用于怀山药零余子中尿囊素成分的含量测定。

[Objective] To determine the allantoin content in Dioscorea opposita Thumb.and bulbil.[Method] With allantoin as the control substance,the content of allantoin was measured using HPLC at the detection wavelength of 224 nm.[Result] The content of allantoin in D.opposita and bulbil was 0.533% and 0.848%,respectively.The average recovery rate of allantoin was 98.35% with relative standard deviations of 1.29%.[Conclusion] The method is simple,easy,accurate and suitable for content determination of allantoin in D.opposita and bulbil.