新型复合功能菌群的构建及其在近海养殖水体BOD微生物传感器中的应用

Isolation of De-Nitrogen Microorganism and Its Application in Seawater's BOD Microbial Biosensors

生化需氧量(BOD)是监测水体中可生物降解的有机物含量的一个重要参数,是目前最常用的水体有机污染综合评价指标之一。随着环境污染问题日益严重,建立和发展快速测定生化需氧量的体系尤其重要。适用于淡水的BOD测定仪已经成型,海水盐度高,BOD浓度低,测定仪样品传输及测量的精度和微生物传感器菌种的耐盐性给构建海水BOD自动测定体系带来困难。从三亚近海养殖水体污染严重的三亚海宝近海养殖专业合作社养殖场水样中分离得到3株能高效降解蛋白质、淀粉和油脂的菌株Bacillus HXY-10、As-pergillus sp.HXY-3和Methylobacterium sp.HXY-7,通过共培养构建能高效去除养殖水体中氨氮和亚硝酸盐氮的固定化复合功能菌群,采用夹膜法制作BOD生物传感器微生物膜。传感器响应电流与水样的BOD含量在2～60 mg/L之间呈线性关系,相关系数为0.992 3。对含有已知BOD含量的水样8次测量,RSD为3.34%,表明BOD传感器检测体系重现性良好,为该复合功能菌群进一步应用于近海养殖水体BOD传感器奠定基础。

The biochemical oxygen demand(BOD) value is a key parameter that can detemine the level of organics,particularly the content of biodegradable organics in water.With the increasingly serious environmental pollution,the system of rapid determination of bio-chemical aerobic quantity is becoming more and more important.The BOD instrument for fresh water has been forming,high salinity of sea water,the low concentration of BOD,the accuracy of samples transmission and measurement,the salt-tolerant of microbial sensors bacteria,is the difficult to build auto-testing instrument for seawater BOD.The actions of three bacteria was isolated from seawaters in Sanya of Hainan Province,China,the compound function bacteria through optimization and formulation with them on high strength organic nitrogen in culturing waters.A microbial biosensing system for the measurement of BOD has been developed using an immobilized mixed culture of Pseudomonas aeruginosa and Klebsiella sp.Together with Clark oxygen(O2) electrode.The linear range of measured BOD content was from 2-60 mg/L,with the correlation coefficient 0.992 3 and RSD 3.34%.Hence,the actions of three bacteria has potential application in developing of seawater's BOD biosensors.