TaNHX1基因的组织特异性表达及生物信息学分析

Tissue-specific Expression and Bioinformatic Analysis of Wheat TaNHX1 Genes

液泡膜蛋白NHX与作物的耐盐、耐旱性紧密相关,其在作物改良中研究较多。通过分析耐旱小麦晋麦47号的NHX1基因表明,其编码的氨基酸序列具有11个跨膜结构,N末端位于膜外,而C末端位于膜内,这与第197位置保守的疏水丙氨酸变为苏氨酸密切相关;且与小麦NHX2有相对较远的进化关系。定量PCR分析表明,小麦中总NHX基因的表达具有组织特异性,根和叶中的表达丰度明显高于茎和穗,这一表达模式可能与植物在耐盐、耐旱过程中根和叶的生理功能相关;而根和叶中表达丰度的差异也可能与不同材料的耐旱性有关。

The function of tonoplast protein NHX was reported that it was closely related to plant drought and salt tolerances in the transgenic crop. In this paper, the wheat （Triticum aestivum） TaNHX1 gene of Jinmai 47 was analyzed to show that the putative amino acid sequence encoded 11 transmembrane structure. Its N-terminal was located in the outer membrane and the C-terminal was loeated in the tonoplast. The result showed that it was related to the conserved hydrophobic alanine in position 197 changed to the threonine. Phylogenetic tree analysis indicated that TaNHX1 had more distant evolutionary relationship with TaNHX2. In addition, expression levels of TaNHXs were analyzed by using real time PCR assays. The gene expression levels of tissue-specific were higher in root and leaf than which in the stem and spike. The result indicated that it might be related to the physiological process of wheat variety responses to salt- and drought-stress. But the diversity of expression levels in root and leave might also be related to the salt- and drought tolerance of different wheat variety.