摘要
研究了津优31号黄瓜适宜的外植体处理方式及诱导不定芽的适宜培养基。结果表明,适宜的外植体处理方式为:保留单片子叶,下胚轴纵切,去除2/3子叶;适宜的不定芽诱导培养基为:MS+TDZ0.005 mg/L+IBA 0.05 mg/L。
Using Jinyou 31 as the material, this experiment studied the suitale explant and the best medium for adventitious buds induction. The results were as followed: the optimum culture medium to induce buds: the medium of MS + 0.005 mg/L TDZ + 0.05 mg/L IBA; The best way of cutting was to keep single cotyledon, to cut hypocotyl longitudinally and remove 2/3 of cotyledon.
出处
《山西农业科学》
2013年第5期438-439,445,共3页
Journal of Shanxi Agricultural Sciences
基金
山西省科技创新计划和科技成果推广计划项目(2007071010)
关键词
黄瓜
组织培养
不定芽诱导
cucumber, tissue culture
adventitious bud induction