一氧化氮在小肠推进运动中的作用

The role of nitric oxide in gastrointestinal motility of mice

目的 观察一氧化氮 (NO)对小鼠小肠推进运动的影响。方法 采用胃内灌注技术 (含 12 %活性炭和4%黄芪胶的炭末胶液灌胃 ) ,计算炭末胶液在小肠内推进距离占小肠全长的百分比 ,以此作为小肠推进速度的指标。观察尾静脉给予NO合成前体L -精氨酸和NO合酶抑制剂L -NAME在小肠推进运动中的作用。结果 正常对照组小肠推进速度为 (70 .6 5± 9.2 2 ) %。尾静脉注射NO合成前体L -精氨酸 (L -Arg)推进速度为 (5 7.33±7.2 5 ) % ,与对照组相比显著减慢 (P <0 .0 1) ;尾静脉注射NO合酶抑制剂L -NG -硝基精氨酸甲酯 (L -NAME)可阻断L -Arg对小肠推进运动的减弱作用 (P <0 .0 1) ,但单独静脉注射L -NAME 12mg kg后 ,小鼠小肠推进运动也明显减慢 ,与对照组相比亦有极其显著性差异 (P <0 .0 1)。结论 NO参与小鼠小肠推进运动的抑制作用 ,小肠推进运动速度除与小肠本身功能有关外 。

Objective To investigate the role of nitric oxide(NO) in gastrointestinal propulsion of mice.?Methods 1 ml of charcoal mixture were infused into the stomachs of the mice, the percentage length of in small intestine travelled by the perfusate within 20 minutes was taken to express the gastrointestinal propulsion speed.?Results The speed of gastrointestinal propulsion in the control and L-Arg animals was (70.65±9.22)% and (57.33±7.25)%, after L-Arg was injected i.v in mice gastrointestinal propulsion reduced 18.85%, compared with those of control (P<0.05).Administration of the NO synthesis blocker L-NAME significantly reduced the effect of NO (P<0.01), but high dose did not. When blockers and L-Arg were administered together, the action of NO was fully blocked.?Conclusion NO may involve in regulating gastrointestinal propulsion motility in mice.