微量快速斑点免疫金银染色法检测囊尾蚴病患者血清抗体的研究

Study on rapid microvolume dot-immunogold-silver staining for detection of serum antibody in patients with cysticercosis

目的 建立一种便捷的囊尾蚴病免疫学诊断方法。方法 在斑点免疫金银染色法 (Dot-IGSS)的基础上 ,待检血清标本原倍使用 ,缩短抗原抗体及与金标二抗的作用时间 ,建立微量快速斑点免疫金银染色法 (RMDot-IGSS)。结果 RMDot-IGSS法检测囊尾蚴病患者血清 96份 ,阳性率为 10 0 % ;检测健康献血员血清 5 0份 ,特异性为 96 % ;检测华支睾吸虫病患者、卫氏并殖吸虫病患者、日本血吸虫病患者血清各 10份 ,均未出现交叉反应 ;但棘球蚴病患者血清 10份有明显的交叉反应。以上结果与Dot-IGSS法检测结果比较 ,无显著性差异 (P >0 .0 5 )。结论 RMDot-IGSS法的敏感性和特异性与Dot-IGSS相似 ,但操作简便快速 。

Objective To set up a simple and convenient immunoassay for the diagnosis of cysticercosis.?Methods A rapid microvolume dot-immunogold-silver staining (RM Dot-IGSS) technique was set up on the basis of the conventional Dot-IGSS by omitting the incubation step to shorten the total reaction time by several hours and by using the sample sera undiluted to elevate the concentration. The results obtained were compared with those of the conventional Dot-IGSS performed simultaneously.?Results All of the 96 confirmed cases of cysticercosis showed positive reactions to RM Dot-IGSS. The negative rate in 50 healthy controls was 96%. The 10 cases of paragonimiasis, 10 clonorchiasis and 10 schistosomiasis,all showed negative reactions. But evident cross-reaction occurred with the sera from the 10 cases of echinococcosis. No significant differences were found in the results between the rapid and the conventional assays ( P >0.05).?Conclusion The sensitivity and specificity of RM Dot-IGSS were similar to those of the conventional Dot-IGSS while the former is simpler, time-saving and more economical.