饲料中维生素C对大菱鲆繁殖性能的影响

Impact of dietary vitamin C on the reproductive performance of turbot Scophthalmus maximus

在基础饲料中分别添加0、800和4800mg/kg的维生素C饲养大菱鲆亲鱼240d,通过比较各处理组亲鱼的生长、性腺指数、相对产卵量、上浮卵率、孵化率、卵径以及各组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,研究维生素C对大菱鲆亲鱼繁殖性能的影响。实验结果表明,各处理组亲鱼生长无显著差异(P>0.05),但随着饲料中维生素C添加量的增加,亲鱼的生长呈上升趋势。800和4800mg/kg组亲鱼相对产卵量显著高于对照组(P<0.05)。随着饲料中维生素C添加量的增加,亲鱼性腺指数、产卵次数、上浮卵率、受精率和孵化率均显著提高(P<0.05),亲鱼卵和组织中维生素C含量反映了饲料中的维生素C含量。虽然各处理组亲鱼的卵径和油球径差异均不显著(P>0.05),但随着饲料中维生素C添加量的增加,亲鱼卵径和油球径呈上升的趋势。肝脏、卵巢和血清中SOD活性随饲料中维生素C添加量的增加而显著升高(P<0.05),而MDA含量则显著下降(P<0.05)。综上所述,大菱鲆亲鱼饲料中添加维生素C,能有效改善大菱鲆的繁殖性能。

An eight-month feeding experiment was conducted to determine the impact of dietary vitamin C on the reproductive performance of turbot Scophthalrnus maximus. The basal diet was supplemented with 0,800 and 4 800 mg/kg vitamin C to formulate three experimental diets. Results showed that the growth of turbot broodstock was improved with the increasing di- etary vitamin C, but no significant difference was observed between control and vitamin C sup- plementation treatments. The egg production of female broodstock fed vitamin C supplemented diets was significantly （P〈0.05） higher than those fed the basal diet. The gonadosomatic index （GSI） ,number of spawns,percentage of buoyant egg,fertilization rate and hatching rate of 800 mg/kg and 4 800 mg/kg treatments were significantly higher with the increase of vitamin Cad-dition in diets（P〈0.05）. Vitamin C concentrations in eggs reflected dietary vitamin C addition. The egg diameter and oil globule diameter of turbot broodstock was improved with the increas- ing dietary vitamin C,but no significant difference was observed between control and vitamin C supplementation treatments. With the increase of dietary vitamin C, the activities of superoxide ismutase （SOD） in tissues were significantly reduced （P〈0.05）,but malonaldehyde （MDA） contents were significantly increased （P〈0.05）. So,it is concluded that vitamin C supplement in the diets can improve the reproductive performance of turbot broodstock.