急性B细胞性白血病CD45阴性细胞的检测

Detection of surface differentiation antigen CD45^- leukemic cells in acute B-lymphoblastic leukemia

目的 探讨细胞表面分化抗原 45阴性 (CD45 -)白血病细胞在急性B细胞性白血病微小残存病变监测中的意义。方法 利用 3种不同荧光标记的单克隆抗体染色及流式细胞仪检测 2 0例急性B淋巴细胞性白血病患儿和 8名正常人骨髓CD10和CD2 2均阳性、CD45阴性或弱阳性(CD10 + CD2 2 + CD45 -或±)表现型细胞。结果 在 2 0例急性B细胞性白血病患儿中 ,均可检测到CD10 + CD2 2 + CD45 -或± 表现型细胞 ,而 8名正常人的CD10 + CD2 2 + CD45 -或± 表现型细胞表现频度均在 0 0 0 1%以下。化疗前 ,急性B细胞性白血病患儿约 75 %骨髓单个核细胞是CD10 + CD2 2 + CD45 -或± 表现型细胞 ,随着化疗进程 ,CD10 + CD2 2 + CD45 -或± 表现型细胞逐渐减少 ,最终消失。结论 CD10 + CD2 2 + CD45 -或± 表现型细胞代表了B细胞性白血病细胞。以CD10 + CD2 2 + CD45 -或± 表现型细胞的变化作为检测B细胞性白血病细胞的指标具有一定的敏感性 ,且可评价化疗疗效。

Objective To discuss the significance of minimal residual disease detection in acute B lymphoblastic leukemia, Surface differentiation antigen CD45 - leukemic cells were detected. Methods Triple color staining and flow cytometry were used to detect CD10 + CD22 + CD45 -or ± cells in patients with acute B lymphoblastic leukemia and normal people. Results CD10 + CD22 + CD45 - or ± cells were found in 20 children with acute B lymphoblastic leukemia. The frequency of CD10 + CD22 + CD45 - or ± cells in 8 normal people was less than 0.001%. About 75% of bone marrow mononuclear cwlls were CD10 +-CD22 +-CD45 - or ± cells in children with acute B lymphoblastic leukemia before chemotherapy. After chemotherapy, the amount of CD10 +-CD22 +-CD45 - or ± cells decreased gradually and finally disappeared.Conclusions B lymphoblastic leukemic cells can be represented by CD10 + CD22 + CD45 -or ± cells. CD10 + CD22 + CD45 - or ± cells are sensitive in detecting B lymphoblastic leukemic cells and evaluating chemotherapy.